Functional reconstitution of RNase P activity from a plastid RNA subunit and a cyanobacterial protein subunit

被引:16
作者
Pascual, A [1 ]
Vioque, A [1 ]
机构
[1] Univ Seville, CSIC, Ctr Invest Cient Isla Cartuja, Inst Bioquim Vegetal & Fotosintesis, E-41092 Seville, Spain
关键词
plastid evolution; RNA catalysis; ribonuclease P; cyanobacteria; Cyanophora cyanelle;
D O I
10.1016/S0014-5793(98)01621-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The plastid (cyanelle) from the Glaucocystophyceae alga Cyanophora paradoxa contains an RNase P RNA subunit (P RNA) similar to the cyanobacterial P RNA. We have synthesized this RNA by in vitro transcription and analyzed its activity in the absence or presence of the RNase P protein subunit (P protein) from Escherichia coli and the cyanobacterium Synechocystis sp. CC 6803, In contrast to the bacterial P RNA, the cyanelle P RNA is not active in the absence of protein in ang of the conditions tested. A functional enzyme could be reconstituted with the Synechocystis protein but not with the E, coli protein. This is the first demonstration of RNase P activity reconstitution from organellar and bacterial subunits. (C) 1999 Federation of European Biochemical Societies.
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页码:7 / 10
页数:4
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