Reversed-phase ion-pair HPLC determination of some water-soluble vitamins in pharmaceuticals

被引：92

作者：

Ivanovic, D

Popovic, A

Radulovic, D

Medenica, M

机构：

[1] Fac Pharm, Dept Pharmaceut Chem & Drug Anal, YU-11000 Belgrade, Yugoslavia

[2] Medifarm, Belgrade, Yugoslavia

[3] Fac Pharm, Dept Physiol Chem, YU-11000 Belgrade, Yugoslavia

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1999年 / 18卷 / 06期

关键词：

reversed-phase ion-pair high-performance liquid chromatography; water-soluble vitamins; pharmaceutical formulations;

D O I：

10.1016/S0731-7085(98)00109-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reversed-phase ion-pair high-performance liquid chromatografic method (RP-IPC) was developed to assay some water-soluble vitamins in solution dosage forms. Vitamins of the B-group B-1, B-2, B-3, and B-6, including vitamin C were determined in Oligovit(R) coated tablets. In Beviplex(R) coated tablets the vitamins B-1, B-2, B-3, B-6 and p-aminobenzoic acid were analysed. Hexanesulphonic acid sodium salt and triethanolamine in water-methanol were used as mobile phase with adjusting pH to 2.8 with orthophosphoric acid. Phenol was used as an internal standard. For quantitative simultaneous analysis of vitamins in pharmaceutical formulations, the method of internal standard was used. All parameters for the validation of the method are given. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：999 / 1004

页数：6

共 19 条

[1]

BACZYK S, 1983, MIKROCHIM ACTA, V1, P437

[2] SENSITIVE ASCORBIC-ACID ASSAY FOR THE ANALYSIS OF PHARMACEUTICAL PRODUCTS AND FRUIT JUICES [J].