Movement of motor and cargo along cilia

被引:127
作者
Orozco, JT [1 ]
Wedaman, KP [1 ]
Signor, D [1 ]
Brown, S [1 ]
Rose, L [1 ]
Scholey, JM [1 ]
机构
[1] Univ Calif Davis, Sect Mol & Cellular Biol, Davis, CA 95616 USA
关键词
D O I
10.1038/19448
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Intraflagellar transport (IFT)1 is important in the formation and maintenance of many cilia, such as the motile cilia that drive the swimming of cells and embryos2, the nodal cilia that generate left-right asymmetry in vertebrate embryos3, and the sensory cilia that detect sensory stimuli in some animals4. The heterotrimeric kinesin-II motor protein drives the anterograde transport of macromolecular complexes, called rafts, along microtubule tracks from the base of the cilium to its distal tip5, whereas cytoplasmic dynein moves the rafts back in the retrograde direction6. We have used fluorescence microscopy to visualize for the first time the intracellular transport of a motor and its cargo in vivo. We observed the anterograde movement of green fluorescent protein (GFP)-labelled kinesin-II motors and IFT rafts within sensory cilia on chemosensory neurons in living Caenorhabditis elegans.
引用
收藏
页码:674 / 674
页数:1
相关论文
共 10 条
  • [1] Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT):: IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons
    Cole, DG
    Diener, DR
    Himelblau, AL
    Beech, PL
    Fuster, JC
    Rosenbaum, JL
    [J]. JOURNAL OF CELL BIOLOGY, 1998, 141 (04) : 993 - 1008
  • [2] NOVEL HETEROTRIMERIC KINESIN-RELATED PROTEIN PURIFIED FROM SEA-URCHIN EGGS
    COLE, DG
    CHINN, SW
    WEDAMAN, KP
    HALL, K
    VUONG, T
    SCHOLEY, JM
    [J]. NATURE, 1993, 366 (6452) : 268 - 270
  • [3] Collet J, 1998, GENETICS, V148, P187
  • [4] Heterotrimeric kinesin-II is required for the assembly of motile 9+2 ciliary axonemes on sea urchin embryos
    Morris, RL
    Scholey, JM
    [J]. JOURNAL OF CELL BIOLOGY, 1997, 138 (05) : 1009 - 1022
  • [5] Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein
    Nonaka, S
    Tanaka, Y
    Okada, Y
    Takeda, S
    Harada, A
    Kanai, Y
    Kido, M
    Hirokawa, N
    [J]. CELL, 1998, 95 (06) : 829 - 837
  • [6] A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT)
    Pazour, GJ
    Wilkerson, CG
    Witman, GB
    [J]. JOURNAL OF CELL BIOLOGY, 1998, 141 (04) : 979 - 992
  • [7] MUTANT SENSORY CILIA IN THE NEMATODE CAENORHABDITIS-ELEGANS
    PERKINS, LA
    HEDGECOCK, EM
    THOMSON, JN
    CULOTTI, JG
    [J]. DEVELOPMENTAL BIOLOGY, 1986, 117 (02) : 456 - 487
  • [8] Intraflagellar transport: The eyes have it
    Rosenbaum, JL
    Cole, DG
    Diener, DR
    [J]. JOURNAL OF CELL BIOLOGY, 1999, 144 (03) : 385 - 388
  • [9] Two heteromeric kinesin complexes in chemosensory neurons and sensory cilia of Caenorhabditis elegans
    Signor, D
    Wedaman, KP
    Rose, LS
    Scholey, JM
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (02) : 345 - 360
  • [10] STARICH TA, 1995, GENETICS, V139, P171