Comparison of the mouse bioassay and enzyme-linked immunosorbent assay procedures for the detection of type A botulinal toxin in food

被引:50
作者
Ferreira, JL
Eliasberg, SJ
Edmonds, P
Harrison, MA
机构
[1] US FDA, Atlanta, GA 30309 USA
[2] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA
[3] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA
关键词
D O I
10.4315/0362-028X-67.1.203
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Samples of chili linked to a foodborne illness outbreak of type A botulism were examined for preformed type A botulinal toxin using two enzyme-linked immunosorbent assay (ELISA) procedures and the mouse bioassay. One of the samples was positive for type A botulinal toxin and three of the samples were negative for type A, B, E, and F botulinal toxins using the three methods. The mouse bioassay indicated that type A toxin was present at the 10,000 minimal lethal dose per gram (MLD per g) of product. The ELISA tests indicated a toxicity of 7,650 MLD per g with one method and 8,350 MLD per g with the other method. The sample toxicity determined by the ELISA was estimated by comparing samples to a standard curve generated with standard type A neurotoxin in casein buffer. The ELISA methods are more rapid than the mouse bioassay, since the toxin type can be determined in I day. The mouse bioassay is more sensitive than the ELISA but usually requires multiple assays to obtain the toxin type and toxicity. Type A culture isolates from the sample were also verified using one ELISA method.
引用
收藏
页码:203 / 206
页数:4
相关论文
共 8 条
  • [1] *AOAC INT, 2000, 97726 AOAC INT
  • [2] Botulinum toxin as a biological weapon - Medical and public health management
    Arnon, SS
    Schechter, R
    Inglesby, TV
    Henderson, DA
    Bartlett, JG
    Ascher, MS
    Eitzen, E
    Fine, AD
    Hauer, J
    Layton, M
    Lillibridge, S
    Osterholm, MT
    O'Toole, T
    Parker, G
    Perl, TM
    Russell, PK
    Swerdlow, DL
    Tonat, K
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2001, 285 (08): : 1059 - 1070
  • [3] Ferreira JL, 2001, J AOAC INT, V84, P1460
  • [4] Ferreira JL, 2001, J AOAC INT, V84, P85
  • [5] FERREIRA JS, 2002, FOOD DRUG ADM LAB IN, V4274, P1
  • [6] KALLURI PC, UNPUB OUTBREAK FOODB
  • [7] PROPERTIES AND USE OF BOTULINUM TOXIN AND OTHER MICROBIAL NEUROTOXINS IN MEDICINE
    SCHANTZ, EJ
    JOHNSON, EA
    [J]. MICROBIOLOGICAL REVIEWS, 1992, 56 (01) : 80 - 99
  • [8] Smith LDS., 1988, BOTULISM ORGANISM IT