Breeding for QX disease resistance negatively selects one form of the defensive enzyme, phenoloxidase, in Sydney rock oysters

被引:34
作者
Bezemer, B
Butt, D
Nell, J
Adlard, R
Raftos, D [1 ]
机构
[1] Macquarie Univ, Dept Biol Sci, N Ryde, NSW 2109, Australia
[2] New S Wales Dept Primary Ind Fisheries, Port Stephens Fisheries Ctr, Nelson Bay, NSW 2315, Australia
[3] Queensland Museum, S Bank, Qld 4101, Australia
基金
澳大利亚研究理事会;
关键词
Saccostrea glomerata; phenoloxidase; QX disease; Marteilia sydneyi; oysters;
D O I
10.1016/j.fsi.2005.08.007
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
QX disease in Sydney rock oysters (Saccostrea glomerata) is caused by the paramyxean protozoan, Marteilia sydneyi. Disease outbreaks occur during summer (January to May) and can result in up to 95% mortality. The New South Wales Department of Primary Industries has been selectively breeding S. glomerata for resistance to QX disease since 1996. Previous work suggests that this breeding program has specifically affected the defensive phenoloxidase enzyme system of oysters. The current study more thoroughly characterises the effect of selection on the different forms of phenoloxidase found in oyster populations. Native polyacrylamide gel electrophoresis (native-PAGE) identified five discrete types of phenoloxidase in non-selected (wild type) and fourth generation QX disease resistant (QXR(4)) oysters. One electrophoretically distinct form of phenoloxidase, POb, is significantly less frequent in resistant oysters when compared to the wild type population. The frequency of POb also decreased in both the wild type and QXR(4) populations over the course of a QX disease outbreak. This suggests that possession of POb makes oysters susceptible to QX disease and that breeding for resistance has resulted in negative selection against this form of phenoloxidase. (C) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:627 / 636
页数:10
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