Phenotypic characteristics of cord blood hemopoietic cells

被引：12

作者：

Dimitriou, H ^{[1
]}

Matsouka, C ^{[1
]}

Perdikoyanni, C ^{[1
]}

Stiakaki, E ^{[1
]}

Bolonaki, I ^{[1
]}

Lydaki, E ^{[1
]}

Koumantakis, E ^{[1
]}

Kalmanti, M ^{[1
]}

机构：

[1] Univ Hosp Heraklion, Dept Pediat Hematol Oncol, Iraklion 71110, Greece

来源：

LEUKEMIA RESEARCH | 1998年 / 22卷 / 08期

关键词：

cord blood; immunophenotype; transplantation; GvHD; CD34(+); childhood malignancies;

D O I：

10.1016/S0145-2126(98)00081-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Bone marrow transplantation is limited due to the lack of HLA-matched donors and to the frequent occurrence of GvHD. Hemopoietic transplants using cord blood cells are being increasingly used in pediatric patients. In this study, the immunophenotypic characteristics of cord blood cells have been investigated by one or two-color flow cytometric analysis. The CB cells were characterized by a low proportion of CD3(+) T-cells, increased CD4/CD8 and CD45RA/CD45RO ratios, minimal expression of HLA-DR, increased proportion of CD5CD19 double positive B-cells, while CD3(-)CD8(+) and CD3(-)CD7(+) subsets, not usually found in adult PB, were detected. These data reflect the immaturity of CB cells as assessed by immunophenotypic analysis suggesting that it could be a valuable alternative source of transplantable hematopoietic progenitor cells and might alleviate some of the problems associated with bone marrow or peripheral blood transplantation. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：755 / 758

页数：4

共 22 条

[1] ACTIVATION OF CORD LYMPHOCYTES-T .2. CELLULAR AND MOLECULAR ANALYSIS OF THE DEFECTIVE RESPONSE INDUCED BY ANTI-CD3 MONOCLONAL-ANTIBODY [J].

BERTOTTO, A ;

GERLI, R ;

LANFRANCONE, L ;

CRUPI, S ;

ARCANGELI, C ;

CERNETTI, C ;

SPINOZZI, F ;

RAMBOTTI, P .

CELLULAR IMMUNOLOGY, 1990, 127 (02) :247-259

[2] GROWTH-CHARACTERISTICS AND EXPANSION OF HUMAN UMBILICAL-CORD BLOOD AND ESTIMATION OF ITS POTENTIAL FOR TRANSPLANTATION IN ADULTS [J].

BROXMEYER, HE ;

HANGOC, G ;

COOPER, S ;

RIBEIRO, RC ;

GRAVES, V ;

YODER, M ;

WAGNER, J ;

VADHANRAJ, S ;

BENNINGER, L ;

RUBINSTEIN, P ;

BROUN, ER .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (09) :4109-4113

[3] HUMAN UMBILICAL-CORD BLOOD AS A POTENTIAL SOURCE OF TRANSPLANTABLE HEMATOPOIETIC STEM PROGENITOR CELLS [J].

BROXMEYER, HE ;

DOUGLAS, GW ;

HANGOC, G ;

COOPER, S ;

BARD, J ;

ENGLISH, D ;

ARNY, M ;

THOMAS, L ;

BOYSE, EA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (10) :3828-3832

[4] Placental and/or umbilical cord blood: An alternative source of hematopoietic stem cells for transplantation [J].

Cairo, MS ;

Wagner, JE .

BLOOD, 1997, 90 (12) :4665-4678

[5] Hematopoietic potential of cryopreserved and ex vivo manipulated umbilical cord blood progenitor cells evaluated in vitro and in vivo [J].

DiGiusto, DL ;

Lee, R ;

Moon, J ;

Moss, K ;

OToole, T ;

Voytovich, A ;

Webster, D ;

Mule, JJ .

BLOOD, 1996, 87 (04) :1261-1271

[6]

Dimitriou H, 1995, BLOOD, V86, P3918

[7]

Gluckman E, 1996, BONE MARROW TRANSPL, V18, P166

[8]

HAN P, 1995, BRIT J HAEMATOL, V89, P733

[9]

HARRIS DT, 1994, BONE MARROW TRANSPL, V14, P545

[10] PHENOTYPIC AND FUNCTIONAL IMMATURITY OF HUMAN UMBILICAL-CORD BLOOD LYMPHOCYTES-T [J].

HARRIS, DT ;

SCHUMACHER, MJ ;

LOCASCIO, J ;

BESENCON, FJ ;

OLSON, GB ;

DELUCA, D ;

SHENKER, L ;

BARD, J ;

BOYSE, EA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10006-10010

← 1 2 3 →