Characterization and electrotransformation of Lactobacillus crispatus isolated from chicken crop and intestine

被引：34

作者：

Beasley, SS

Takala, TM

Reunanen, J

Apajalahti, J

Saris, PEJ ^{[1
]}

机构：

[1] Univ Helsinki, Div Microbiol, Dept Appl Chem & Microbiol, FIN-00014 Helsinki, Finland

[2] Danisco Innovat, Enteromix Res, FIN-02460 Kantvik, Finland

来源：

POULTRY SCIENCE | 2004年 / 83卷 / 01期

关键词：

bioengineering; lactic acid bacteria; poultry; probiotic improvement;

D O I：

10.1093/ps/83.1.45

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Lactic acid bacteria originating in the intestine have recently undergone intensive study for their potential probiotic properties. Here partial 16S rRNA gene sequencing of 8 Lactobacillus strains proved them to be Lactobacillus crispatus. Fatty acid analysis confirmed strains being closely related. These strains and type strain ATCC33820 were characterized for genetic engineering potential, thus determining aerobic growth, erythromycin sensitivity, and glycine tolerance. Out of 5 plasmids, a 2.9-kb plasmid (pLEB579) was successfully introduced into 4 chicken-originated wild-type L. crispatus strains. Transformation frequency was approximately 30 transformants per microgram of DNA, the first reported electrotransformation into chicken-originated L. crispatus. In spite of its low frequency, transformation enables bioengineering of these strains to improve the probiotic function in feed adsorption, chicken health, and food safety.

引用

页码：45 / 48

页数：4

共 34 条

[1] COMPETITIVE-EXCLUSION OF CAMPYLOBACTERS FROM POULTRY WITH K-BACTERIA AND BROILACT (R) [J].