A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor

被引：13

作者：

Garden, SR

Doellgast, GJ

Killham, KS

Strachan, NJC

机构：

[1] Univ Aberdeen, Dept Plant Soil Sci, Sch Biol Sci, Aberdeen AB24 3UU, Scotland

[2] Amplistar Inc, Winston Salem, NC 27101 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2004年 / 19卷 / 07期

关键词：

fibre optic sensor; evanescent wave sensor; thrombin; blood coagulation; fluorescent fibrinogen;

D O I：

10.1016/j.bios.2003.08.002

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A fibre optic evanescent wave sensor is used for the rapid detection of thrombin. Coagulation of solution phase fluorescently labelled fibrinogen to unlabelled fibrinogen bound to the surface of the fibre optic is observed in real time by the evanescent wave sensor. Thrombin concentrations down to 0.01 NIH ml(-1) are detectable within 5 min. The potential application of this technique for rapid amplified immunosensing is discussed. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：737 / 740

页数：4

共 18 条

[1]

BIGGS R, 1962, HUMAN BLOOD COAGULAT

[2] ENZYME-LINKED COAGULATION ASSAY .4. SENSITIVE SANDWICH ENZYME-LINKED IMMUNOSORBENT ASSAYS USING RUSSELLS VIPER VENOM FACTOR-X ACTIVATOR ANTIBODY CONJUGATES [J].