Differential expression of PPAR gamma 1 and gamma 2 isoforms in human adipose tissue

被引：50

作者：

Yanase, T ^{[1
]}

Yashiro, T ^{[1
]}

Takitani, K ^{[1
]}

Kato, S ^{[1
]}

Taniguchi, S ^{[1
]}

Takayanagi, R ^{[1
]}

Nawata, H ^{[1
]}

机构：

[1] UNIV TOKYO,INST MOL & CELLULAR BIOSCI,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 233卷 / 02期

关键词：

D O I：

10.1006/bbrc.1997.6446

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One of the essential factors for adipogenesis, peroxisome proliferator activated receptor gamma (PPAR gamma), is classified into two isoforms, gamma 1 and gamma 2 encoded by a single PPAR gamma gene, To examine the mode of expressions of both the PPAR gamma 1 and gamma 2 isoforms in human adipose tissue, we cloned a partial cDNA of the human PPAR gamma 2 (hPPAR gamma 2) from a human adipose tissue cDNA library. The sequence encoded an additional 28 amino acids amino-terminal to the first ATG codon of hPPAR gamma 1. The simultaneous quantitation of hPPAR gamma 1 and gamma 2 mRNA in subcutaneous or visceral (mesenteric) fat tissue from 10 different individuals was performed by an RNase protection assay and revealed a relatively higher expression of gamma 1 than gamma 2 in all specimenes examined. (C) 1997 Academic Press.

引用

页码：320 / 324

页数：5

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