Development of STS markers closely linked to the Ppd-H1 photoperiod response gene of barley (Hordeum vulgare L.)

被引：24

作者：

Decousset, L ^{[1
]}

Griffiths, S ^{[1
]}

Dunford, RP ^{[1
]}

Pratchett, N ^{[1
]}

Laurie, DA ^{[1
]}

机构：

[1] John Innes Ctr, Norwich NR4 7UH, Norfolk, England

来源：

THEORETICAL AND APPLIED GENETICS | 2000年 / 101卷 / 08期

关键词：

amplified fragment length polymorphism (AFLP); barley (Hordeum vulgare); photoperiod; mapping; Ppd-H1; sequence tagged site (STS);

D O I：

10.1007/s001220051598

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A BC2 population of 353 plants segregating for the Ppd-HI photoperiod response gene was developed from a cross between the winter barley 'Igri' and the spring barley 'Triumph. Bulk segregant analysis identified six AFLP markers closely linked to the Ppd-H1 gene and three strongly amplified AFLP bands that mapped 0.8-cM distal, 0.6-cM proximal and 2.3-cm proximal to Ppd-H1 were cloned and sequenced. Southern-blot analysis showed that the cloned fragments were single-copy sequences in 'Igri', the variety from which they were derived. Two of the sequences were absent from 'Triumph' while the third detected a single-copy sequence. The cloned fragments were used to design specific sequence tagged site (STS) primer pairs to assist in the construction of a high-resolution map of the Ppd-H1 region.

引用

页码：1202 / 1206

页数：5

共 11 条

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