SbcCD regulation and localization in Escherichia coli

被引：14

作者：

Darmon, Elise ^{[1
]}

Lopez-Vernaza, Manuel A. ^{[1
]}

Helness, Anne C. ^{[1
]}

Borking, Amanda ^{[1
]}

Wilson, Emily ^{[1
]}

Thacker, Zubin ^{[1
]}

Wardrope, Laura ^{[1
]}

Leach, David R. F. ^{[1
]}

机构：

[1] Univ Edinburgh, Inst Cell Biol, Kings Bldg, Edinburgh EH9 3JR, Midlothian, Scotland

来源：

JOURNAL OF BACTERIOLOGY | 2007年 / 189卷 / 18期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1128/JB.00489-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The SbcCD complex and its homologues play important roles in DNA repair and in the maintenance of genome stability. In Escherichia coli, the in vitro functions of SbcCD have been well characterized, but its exact cellular role remains elusive. This work investigates the regulation of the sbcDC operon and the cellular localization of the SbcC and SbcD proteins. Transcription of the sbcDC operon is shown to be dependent on starvation and RpoS protein. Overexpressed SbcC protein forms foci that colocalize with the replication factory, while overexpressed SbcD protein is distributed through the cytoplasm.

引用

页码：6686 / 6694

页数：9

共 31 条

[1] Large-scale identification of protein-protein interaction of Escherichia coli K-12 [J].

Arifuzzaman, M ;

Maeda, M ;

Itoh, A ;

Nishikata, K ;

Takita, C ;

Saito, R ;

Ara, T ;

Nakahigashi, K ;

Huang, HC ;

Hirai, A ;

Tsuzuki, K ;

Nakamura, S ;

Altaf-Ul-Amin, M ;

Oshima, T ;

Baba, T ;

Yamamoto, N ;

Kawamura, T ;

Ioka-Nakamichi, T ;

Kitagawa, M ;

Tomita, M ;

Kanaya, S ;

Wada, C ;

Mori, H .

GENOME RESEARCH, 2006, 16 (05) :686-691

[2] The complete genome sequence of Escherichia coli K-12 [J].

Blattner, FR ;

Plunkett, G ;

Bloch, CA ;

Perna, NT ;

Burland, V ;

Riley, M ;

ColladoVides, J ;

Glasner, JD ;

Rode, CK ;

Mayhew, GF ;

Gregor, J ;

Davis, NW ;

Kirkpatrick, HA ;

Goeden, MA ;

Rose, DJ ;

Mau, B ;

Shao, Y .

SCIENCE, 1997, 277 (5331) :1453-+

[3] Towards single-copy gene expression systems making gene cloning physiologically relevant:: Lambda InCh, a simple Escherichia coli plasmid-chromosome shuttle system [J].

Boyd, D ;

Weiss, DS ;

Chen, JC ;

Beckwith, J .

JOURNAL OF BACTERIOLOGY, 2000, 182 (03) :842-847

[4] Interaction network containing conserved and essential protein complexes in Escherichia coli [J].

Butland, G ;

Peregrín-Alvarez, JM ;

Li, J ;

Yang, WH ;

Yang, XC ;

Canadien, V ;

Starostine, A ;

Richards, D ;

Beattie, B ;

Krogan, N ;

Davey, M ;

Parkinson, J ;

Greenblatt, J ;

Emili, A .

NATURE, 2005, 433 (7025) :531-537

[5] A monomeric red fluorescent protein [J].

Campbell, RE ;

Tour, O ;

Palmer, AE ;

Steinbach, PA ;

Baird, GS ;

Zacharias, DA ;

Tsien, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (12) :7877-7882

[6] ESCHERICHIA-COLI SBCC MUTANTS PERMIT STABLE PROPAGATION OF DNA REPLICONS CONTAINING A LONG PALINDROME [J].

CHALKER, AF ;

LEACH, DRF ;

LLOYD, RG .

GENE, 1988, 71 (01) :201-205

[7] Tethering on the brink: the evolutionarily conserved Mre11-Rad50 complex [J].

Connelly, JC ;

Leach, DRF .

TRENDS IN BIOCHEMICAL SCIENCES, 2002, 27 (08) :410-418

[8]

Cromie GA, 2000, GENETICS, V154, P513

[9] Pre-replication assembly of E-coli replisome components [J].

den Blaauwen, Tanneke ;

Aarsman, Mirjam E. G. ;

Wheeler, Linda J. ;

Nanninga, Nanne .

MOLECULAR MICROBIOLOGY, 2006, 62 (03) :695-708

[10] Signal transduction and regulatory mechanisms involved in control of the σS (RpoS) subunit of RNA polymerase [J].

Hengge-Aronis, R .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2002, 66 (03) :373-+

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