Substrate stress relaxation regulates cell spreading

被引：727

作者：

Chaudhuri, Ovijit ^{[1
,2
,3
]}

Gu, Luo ^{[1
,2
]}

Darnell, Max ^{[1
,2
]}

Klumpers, Darinka ^{[1
,2
,4
]}

Bencherif, Sidi A. ^{[1
,2
]}

Weaver, James C. ^{[2
]}

Huebsch, Nathaniel ^{[1
,5
]}

Mooney, David J. ^{[1
,2
]}

机构：

[1] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[2] Harvard Univ, Wyss Inst Biol Inspired Engn, Cambridge, MA 02138 USA

[3] Stanford Univ, Dept Mech Engn, Stanford, CA 94305 USA

[4] Vrije Univ Amsterdam Med Ctr, Res Inst MOVE, Dept Orthoped Surg, NL-1081 HV Amsterdam, Netherlands

[5] Gladstone Inst Cardiovasc Dis, San Francisco, CA 94158 USA

来源：

NATURE COMMUNICATIONS | 2015年 / 6卷

关键词：

EXTRACELLULAR-MATRIX; TRACTION; TISSUE; MECHANOTRANSDUCTION; STIFFNESS; ADHESION; BEHAVIOR; INTERFACE; FORCES; FATE;

D O I：

10.1038/ncomms7365

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Studies of cellular mechanotransduction have converged upon the idea that cells sense extracellular matrix (ECM) elasticity by gauging resistance to the traction forces they exert on the ECM. However, these studies typically utilize purely elastic materials as substrates, whereas physiological ECMs are viscoelastic, and exhibit stress relaxation, so that cellular traction forces exerted by cells remodel the ECM. Here we investigate the influence of ECM stress relaxation on cell behaviour through computational modelling and cellular experiments. Surprisingly, both our computational model and experiments find that spreading for cells cultured on soft substrates that exhibit stress relaxation is greater than cells spreading on elastic substrates of the same modulus, but similar to that of cells spreading on stiffer elastic substrates. These findings challenge the current view of how cells sense and respond to the ECM.

引用

页数：7

共 31 条

[1]

Activation of integrin function by nanopatterned adhesive interfaces [J].