Gene expression and characterization of isoprene synthase from Populus alba

被引：100

作者：

Sasaki, K ^{[1
]}

Ohara, K ^{[1
]}

Yazaki, K ^{[1
]}

机构：

[1] Kyoto Univ, Lab Plant Gene Express, Res Inst Sustainable Humanosphere, Uji 6110011, Japan

来源：

FEBS LETTERS | 2005年 / 579卷 / 11期

关键词：

gene expression; heterologous expression; isoprene synthase; subcellular localization; substrate specificity; Populus alba;

D O I：

10.1016/j.febslet.2005.03.066

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isoprene synthase cDNA from Populus alha (PaIspS) was isolated by RT-PCR. This PaIspS mRNA, which was predominantly observed in the leaves, was strongly induced by heat stress and continuous light irradiation, and was substantially decreased in the dark, suggesting that isoprene emission was regulated at the transcriptional level. The subcellular localization of PaIspS protein with green fluorescent protein fusion was shown to be in plastids. PaIspS expressed in Escherichia coli was characterized enzymatically: it had an optimum pH of approximately 8.0, and an optimum temperature 40 degrees C. Its preference for divalent cations for its activity was also studied. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2514 / 2518

页数：5

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