Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells

被引：1384

作者：

Mittelbrunn, Maria ^{[1
]}

Gutierrez-Vazquez, Cristina ^{[1
]}

Villarroya-Beltri, Carolina ^{[1
]}

Gonzalez, Susana ^{[1
]}

Sanchez-Cabo, Fatima ^{[1
]}

Angel Gonzalez, Manuel ^{[1
]}

Bernad, Antonio ^{[1
]}

Sanchez-Madrid, Francisco ^{[1
,2
]}

机构：

[1] Ctr Nacl Invest Cardiovasc, Madrid 328029, Spain

[2] Hosp Univ Princesa, Inst Invest Sanitaria Princesa, Serv Inmunol, Madrid 28006, Spain

来源：

NATURE COMMUNICATIONS | 2011年 / 2卷

关键词：

MICROTUBULE-ORGANIZING CENTER; AUREUS ENTEROTOXIN-B; IMMUNOLOGICAL SYNAPSE; INTERCELLULAR TRANSFER; HORIZONTAL TRANSFER; IMMUNE-SYSTEM; ACTIVATION; PROTEIN; MICROVESICLES; TRAFFICKING;

D O I：

10.1038/ncomms1285

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The immune synapse is an exquisitely evolved means of communication between T cells and antigen-presenting cells (APCs) during antigen recognition. Recent evidence points to the transfer of RNA via exosomes as a novel mode of intercellular communication. Here we show that exosomes of T, B and dendritic immune cells contain microRNA (miRNA) repertoires that differ from those of their parent cells. We investigate whether miRNAs are exchanged during cognate immune interactions, and demonstrate the existence of antigen-driven unidirectional transfer of miRNAs from the T cell to the APC, mediated by the delivery of CD63(+) exosomes on immune synapse formation. Inhibition of exosome production by targeting neutral sphingomyelinase-2 impairs transfer of miRNAs to APCs. Moreover, miRNAs transferred during immune synapsis are able to modulate gene expression in recipient cells. Thus, our results support a mechanism of cellular communication involving antigen-dependent, unidirectional intercellular transfer of miRNAs by exosomes during immune synapsis.

引用

页数：10

共 60 条

[1] Vesicle Traffic to the Immunological Synapse: A Multifunctional Process Targeted by Lymphotropic Viruses [J].