Molecular cloning, genomic mapping, and expression of two secretor blood group α(1,2)fucosyltransferase genes differentially regulated in mouse uterine epithelium and gastrointestinal tract

被引:45
作者
Domino, SE
Zhang, L
Lowe, JB
机构
[1] Univ Michigan, Dept Obstet & Gynecol, Sch Med, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Howard Hughes Med Inst, Sch Med, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Pathol, Sch Med, Ann Arbor, MI 48109 USA
关键词
D O I
10.1074/jbc.M100735200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fucosylated oligosaccharides have been proposed to be involved in multiple cell-cell interactions, including mouse blastocyst adhesion and intestine-microbe interactions. To begin to define the regulation and function of terminal alpha (1,2)fucosylated carbohydrates in these and other tissues, we isolated and characterized a 85-kilobase (kb) genomic region of mouse chromosome 7, 23.2 centimorgans analogous to human chromosome 19q13.3 that encodes three alpha (1,2) fucosyltransferases. Gene-specific DNA probes from the open reading frames of the mouse fucosyltransferase genes corresponding to human FUT1, FUT2, and SEC1 demonstrate distinct tissue-specific expression patterns by Northern blot analyses. Flow cytometry profiles of cultured cells transfected with DNA segments containing the open reading frames of the mouse genes confirm that, each encodes an alpha (1,2)fucosyltransferase. In uterus and colon, a 3.3-kb FUT2 mRNA represents the major fucosyltransferase gene expressed. Steady-state FUT2 mRNA levels are cyclically regulated during the estrus cycle, increasing 10-fold from early diestrus to a relative maximum in proestrus. In contrast, SEC1 and FUT1 do not show prominently regulated expression in uterus, FUT2 expression localizes to luminal uterine epithelium by in situ hybridization, implying that this gene determines expression of cell surface Fuc alpha1 --> 2Gal beta epitopes proposed to mediate blastocyst adhesion.
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页码:23748 / 23756
页数:9
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