The mucin degrader Akkermansia muciniphila is an abundant resident of the human intestinal tract

被引：522

作者：

Derrien, Muriel ^{[1
]}

Collado, M. Carmen ^{[2
,3
]}

Ben-Amor, Kaouther ^{[1
]}

Salminen, Seppo ^{[2
,3
]}

de Vos, Willem M. ^{[1
]}

机构：

[1] Microbiol Lab, NL-6703 HB Wageningen, Netherlands

[2] Univ Turku, FI-20014 Turku, Finland

[3] Univ Helsinki, Dept Vet Basic Sci, FI-00014 Helsinki, Finland

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 05期

关键词：

D O I：

10.1128/AEM.01226-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A 16S rRNA-targeted probe, MUC-1437, was designed and validated in order to determine the presence and numbers of cells of Akkermansia muciniphila, a mucin degrader, in the human intestinal tract. As determined by fluorescent in situ hybridization, A. muciniphild accounted more than 1% of the total fecal cells and was shown to be a common bacterial component of the human intestinal tract.

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页码：1646 / 1648

页数：3

相关论文

共 19 条

[1] MUCUS - A PROTECTIVE SECRETION OF COMPLEXITY [J].

ALLEN, A .

TRENDS IN BIOCHEMICAL SCIENCES, 1983, 8 (05) :169-173

[2] The oligonucleotide probe database [J].

Alm, EW ;

Oerther, DB ;

Larsen, N ;

Stahl, DA ;

Raskin, L .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (10) :3557-3559

[3] FLUORESCENT-OLIGONUCLEOTIDE PROBING OF WHOLE CELLS FOR DETERMINATIVE, PHYLOGENETIC, AND ENVIRONMENTAL-STUDIES IN MICROBIOLOGY [J].

AMANN, RI ;

KRUMHOLZ, L ;

STAHL, DA .

JOURNAL OF BACTERIOLOGY, 1990, 172 (02) :762-770

[4] The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis [J].

Cole, JR ;

Chai, B ;

Farris, RJ ;

Wang, Q ;

Kulam, SA ;

McGarrell, DM ;

Garrity, GM ;

Tiedje, JM .

NUCLEIC ACIDS RESEARCH, 2005, 33 :D294-D296

[5] Intestinal integrity and Akkermansia muciniphila, a mucin-degrading member of the intestinal microbiota present in infants, adults, and the elderly [J].

Collado, M. Carmen ;

Derrien, Muriel ;

Isolauri, Erika .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (23) :7767-7770

[6] Akkermansia muciniphila gen. nov., sp nov., a human intestinal mucin-degrading bacterium [J].

Derrien, M ;

Vaughan, EE ;

Plugge, CM ;

de Vos, WM .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2004, 54 :1469-1476

[7] Diversity of the human intestinal microbial flora [J].

Eckburg, PB ;

Bik, EM ;

Bernstein, CN ;

Purdom, E ;

Dethlefsen, L ;

Sargent, M ;

Gill, SR ;

Nelson, KE ;

Relman, DA .

SCIENCE, 2005, 308 (5728) :1635-1638

[8] Fecal microbial diversity in a strict vegetarian as determined by molecular analysis and cultivation [J].

Hayashi, H ;

Sakamoto, M ;

Benno, Y .

MICROBIOLOGY AND IMMUNOLOGY, 2002, 46 (12) :819-831

[9] Verrucomicrobia div. nov., a new division of the bacteria containing three new species of Prosthecobacter [J].

Hedlund, BP ;

Gosink, JJ ;

Staley, JT .

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 1997, 72 (01) :29-38

[10]

Hold GL, 2002, FEMS MICROBIOL ECOL, V39, P33, DOI 10.1111/j.1574-6941.2002.tb00904.x