Seasonal detection of pear decline phytoplasma by nested-PCR in different pear cultivars

被引：24

作者：

Garcia-Chapa, M ^{[1
]}

Medina, V

Viruel, MA

Laviña, A

Batlle, A

机构：

[1] Inst Rec & Tecnol Agroalimentaries, Dept Proteccio Vegetal, Barcelona 08348, Spain

[2] Univ Lleida, IRTA, Lleida 25006, Spain

[3] Inst Rec & Tecnol Agroalimentaries, Dept Genet, Barcelona 08348, Spain

来源：

PLANT PATHOLOGY | 2003年 / 52卷 / 04期

关键词：

detection; grafting; nested-PCR; Pyrus communis; transmission;

D O I：

10.1046/j.1365-3059.2003.00868.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Seasonal detection of pear decline phytoplasma was studied in three pear cultivars: Bartlett, Limonera and Blanquilla. Samples from 43 infected trees were collected monthly over 2 years and analysed by nested PCR. The three cultivars each showed a different pattern of phytoplasma detection. The maximum detection rate of pear decline phytoplasma occurred in December in the three orchards, and it remained high throughout the winter months. In spring, when new buds appeared and sap was produced, the detection rate decreased. Leaf midribs, buds and stems were compared to determine which sample was more reliable for phytoplasma detection. The best indicators were stems. The presence of phytoplasma in sieve tubes during the dormant season was determined by grafting. The results suggest that phytoplasmas could overwinter in shoots, with the implication that vegetative propagation during this period could also disseminate the disease.

引用

页码：513 / 520

页数：8

共 34 条

[1] DETECTION OF DNA OF PLANT PATHOGENIC MYCOPLASMALIKE ORGANISMS BY A POLYMERASE CHAIN-REACTION THAT AMPLIFIES A SEQUENCE OF THE 16S RNA GENE [J].

AHRENS, U ;

SEEMULLER, E .

PHYTOPATHOLOGY, 1992, 82 (08) :828-832

[2]

[Anonymous], 1960, U CALIF PUBL BOT

[3] Pear decline in Spain [J].

Avinent, L ;

Llacer, G ;

Almacellas, J ;

Tora, R .

PLANT PATHOLOGY, 1997, 46 (05) :694-698

[4]

BATLLE A, 2001, P 5 C EUR FDN PLANT, P196

[5]

BLODGETT EARLE C., 1963, PLANT DIS REPTR, V47, P89

[6] HISTOPATHOLOGY OF PHLOEM NECROSIS IN ULMUS AMERICANA [J].

BRAUN, EJ ;

SINCLAIR, WA .

PHYTOPATHOLOGY, 1976, 66 (05) :598-607

[7] CLONED DNA PROBES FOR DETECTION OF GRAPEVINE FLAVESCENCE DOREE MYCOPLASMA-LIKE ORGANISM (MLO) [J].

DAIRE, X ;

BOUDONPADIEU, E ;

BERVILLE, A ;

SCHNEIDER, B ;

CAUDWELL, A .

ANNALS OF APPLIED BIOLOGY, 1992, 121 (01) :95-103

[8] PARRYS DISEASE OF PEARS IS SIMILAR TO PEAR DECLINE AND IS ASSOCIATED WITH MYCOPLASMA-LIKE ORGANISMS TRANSMITTED BY CACOPSYLLA-PYRICOLA [J].

DAVIES, DL ;

GUISE, CM ;

CLARK, MF ;

ADAMS, AN .

PLANT PATHOLOGY, 1992, 41 (02) :195-203

[9]

Del Serrone P., 1998, Journal of Plant Pathology, V80, P53

[10] AMPLIFICATION OF 16S RIBOSOMAL-RNA GENES FROM CULTURABLE AND NONCULTURABLE MOLLICUTES [J].

DENG, SJ ;

HIRUKI, C .

JOURNAL OF MICROBIOLOGICAL METHODS, 1991, 14 (01) :53-61

← 1 2 3 4 →