Regulation of adiponectin receptor expression in human liver and a hepatocyte cell line

被引:45
作者
Shimizu, Akiko
Takamura, Toshinari [1 ]
Matsuzawa, Naoto
Nakamura, Seiji
Nabemoto, Satoko
Takeshita, Yurnie
Misu, Hirofumi
Kurita, Seiichiro
Sakurai, Masaru
Yokoyama, Masayoshi
Zen, Yoh
Sasaki, Motoko
Nakanuma, Yasuni
Kaneko, Shuichi
机构
[1] Kanazawa Univ, Dept Dis Control & Homeostasis, Grad Sch Med Sci, Kanazawa, Ishikawa 9208641, Japan
[2] Kanazawa Univ, Dept Human Pathol, Grad Sch Med Sci, Kanazawa, Ishikawa 9208641, Japan
来源
METABOLISM-CLINICAL AND EXPERIMENTAL | 2007年 / 56卷 / 11期
关键词
D O I
10.1016/j.metabol.2007.06.013
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Nonalcoholic fatty liver disease (NAFLD) is closely associated with obesity. An adipocyte-derived hormone, adiponectin, may play a role in the pathophysiolo-gy of NAFLD through insulin-sensitizing and antifibrotic effects. We found that hepatic expression of adiponectin receptor AdipoR2, but not AdipoR1, was down-regulated in 14 patients with NAFLD compared with 7 patients with a normal liver (P <.05). To investigate the significance of the adiponectin system in obesity and NAFLD, we examined the regulation of AdipoR2 expression in a nonmalienant human hepatocyte cell line, the THLE-5b cells. Insulin down-regulated the levels of AdipoR2 messenger RNA (mRNA) and protein, whereas an adipocyrokine, turnor necrosis factor g., up-regulated them. A thiazolidinedione, pioglitazone, up-regulated the expression of AdipoR2 mRNA and protein in THLE-5b cells. The AdipoR2 mRNA level was decreased in fatty THLE-5b cells induced by coincubating with fatty acids. These findings suggest that down-regulation of AdipoR2 in the liver caused by hypermsulinernia and steatosis may play a role in the development of NAFLD. (C) 2007 Elsevier hic. All rights reserved.
引用
收藏
页码:1478 / 1485
页数:8
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