Circadian rhythm gene regulation in the housefly Musca domestica

被引:40
作者
Codd, Veryan
Dolezel, David
Stehlik, Jan
Piccin, Alberto
Garner, Karen J.
Racey, Seth N.
Straatman, Kornelis R.
Louis, Edward J.
Costa, Rodolfo
Sauman, Ivo
Kyriacou, Charalambos P.
Rosato, Ezio
机构
[1] Univ Leicester, Dept Genet, Leicester LE1 7RH, Leics, England
[2] Univ Leicester, Dept Biol, Leicester LE1 7RH, Leics, England
[3] Acad Sci Czech Republ, Inst Entomol, CR-37005 Ceske Budejovice, Czech Republic
[4] Univ S Bohemia, Fac Biol Sci, Ceske Budejovice 37005, Czech Republic
[5] Univ Padua, Dept Biol, I-35131 Padua, Italy
基金
英国惠康基金;
关键词
D O I
10.1534/genetics.107.079160
中图分类号
Q3 [遗传学];
学科分类号
071007 [遗传学]; 090102 [作物遗传育种];
摘要
The circadian mechanism appears remarkably conserved between Drosophila and mammals, with basic underlying negative and positive feedback loops, cycling gene products, and temporally regulated nuclear transport involving a few key proteins. One of these negative regulators is PERIOD, which in Drosophila shows very similar temporal and spatial regulation to TIMELESS. Surprisingly, we observe that in the housefly, Musca domestica, PER does not cycle in Western blots of head extracts, in contrast to the TIM protein. Furthermore, inmulnocytochemical (ICC) localization using enzymatic staining procedures reveals that PER is not localized to the nucleus of any neurons within the brain at any circadian time, as recently observed for several nondipteran insects. However, with confocal analysis, immunofluorescence reveals a very different picture and provides an initial comparison of PER/TIM-containing cells in Musca and Drosophila, which shows some significant differences, but many similarities. Thus, even in closely related Diptera, there is considerable evolutionary flexibility in the number and spatial organization of clock cells and, indeed, in the expression patterns of clock products in these cells, although the underlying framework is similar.
引用
收藏
页码:1539 / 1551
页数:13
相关论文
共 57 条
[1]
A role for CK2 in the Drosophila circadian oscillator [J].
Akten, B ;
Jauch, E ;
Genova, GK ;
Kim, EY ;
Edery, I ;
Raabe, T ;
Jackson, FR .
NATURE NEUROSCIENCE, 2003, 6 (03) :251-257
[2]
Ashmore LJ, 2003, J NEUROSCI, V23, P7810
[3]
PDP1ε functions downstream of the circadian oscillator to mediate behavioral rhythms [J].
Benito, Juliana ;
Zheng, Hao ;
Hardin, Paul E. .
JOURNAL OF NEUROSCIENCE, 2007, 27 (10) :2539-2547
[4]
Constructing a feedback loop with circadian clock molecules from the silkmoth, Antheraea pernyi [J].
Chang, DC ;
McWatters, HG ;
Williams, JA ;
Gotter, AL ;
Levine, JD ;
Reppert, SM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (40) :38149-38158
[5]
Circadian regulation of gene expression systems in the Drosophila head [J].
Claridge-Chang, A ;
Wijnen, H ;
Naef, F ;
Boothroyd, C ;
Rajewsky, N ;
Young, MW .
NEURON, 2001, 32 (04) :657-671
[6]
Drosophila CRYPTOCHROME is a circadian transcriptional repressor [J].
Collins, B ;
Mazzoni, EO ;
Stanewsky, R ;
Blau, J .
CURRENT BIOLOGY, 2006, 16 (05) :441-449
[7]
Even a stopped clock tells the right time twice a day:: circadian timekeeping in Drosophila [J].
Collins, Ben ;
Blau, Justin .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2007, 454 (05) :857-867
[8]
Disruption of cryptochrome partially restores circadian rhythmicity to the arrhythmic period mutant of Drosophila [J].
Collins, BH ;
Dissel, S ;
Gaten, E ;
Rosato, E ;
Kyriacou, CP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (52) :19021-19026
[9]
vrille, Pdp1, and dClock form a second feedback loop in the Drosophila circadian clock [J].
Cyran, SA ;
Buchsbaum, AM ;
Reddy, KL ;
Lin, MC ;
Glossop, NRJ ;
Hardin, PE ;
Young, MW ;
Storti, RV ;
Blau, J .
CELL, 2003, 112 (03) :329-341
[10]
A constitutively active cryptochrome in Drosophila melanogaster [J].
Dissel, S ;
Codd, V ;
Fedic, R ;
Garner, KJ ;
Costa, R ;
Kyriacou, CP ;
Rosato, E .
NATURE NEUROSCIENCE, 2004, 7 (08) :834-840