A water-soluble azobenzene cross-linker for photocontrol of peptide conformation

被引：77

作者：

Zhang, ZH

Burns, DC

Kumita, JR

Smart, OS

Woolley, GA

机构：

[1] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada

[2] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

来源：

BIOCONJUGATE CHEMISTRY | 2003年 / 14卷 / 04期

关键词：

D O I：

10.1021/bc0340161

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have designed and synthesized a water-soluble, sulfonated version of an azobenzene-based thiol-reactive cross-linker that can be introduced into peptides and proteins and act as a conformational photoswitch. The sulfonated compound is shown to effect a similar degree of conformational control on a model peptide helix system, as its nonsulfonated counterpart but can be introduced without the need for any organic cosolvent. The sulfonated azobenzene cross-linker thus expands the range of proteins to which photocontrol can be applied.

引用

页码：824 / 829

页数：6

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