Development of a nerve scaffold using a tendon chitosan tube

被引：61

作者：

Itoh, S

Suzuki, M

Yamaguchi, I

Takakuda, K

Kobayashi, H

Shinomiya, K

Tanaka, J

机构：

[1] Tokyo Med & Dent Univ, Div Mol Tissue Engn, Tokyo, Japan

[2] Tokyo Med & Dent Univ, Dept Plast Surg, Tokyo, Japan

[3] Taki Chem Co, Kako, Hyogo, Japan

[4] NIMS, Ctr Biomat, Ibaraki, Japan

来源：

ARTIFICIAL ORGANS | 2003年 / 27卷 / 12期

关键词：

tendon chitosan tube; nerve scaffold; configuration of tube cross-section; laminin; laminin peptide; nerve regeneration;

D O I：

10.1111/j.1525-1594.2003.07208.x

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Bridge grafting (15 mm) into the sciatic nerve of SD rats was carried out using tendon chitosan tubes having either a circular or triangular cross-section, as well as triangular tubes combined with laminin, CDPGYIGSR, or CSRARKQAASIKVAVSAD (n = 15 in each group). As a control, isografting (15 mm) was carried out in the SD rats (n = 7). Specimens were taken after 1, 2, 4, 6, and 8 weeks for histology, and nerve regeneration was evaluated electro-physiologically and histologically after 12 weeks. The mechanical strength of triangular tubes was found to be higher than circular tubes, and the inner volume of a triangular tube tends to be larger than in circular tubes. Nerve tissue regeneration along the tube wall was found in both the laminin and laminin peptide groups. According to the result of percentage neural tissue in relation to evoked action potentials, the consecutive treatments of YIGSR and IKVAV was found to match the effectiveness of intact laminin.

引用

页码：1079 / 1088

页数：10

共 28 条

[1] Antibodies directed against the beta 1-integrin subunit and peptides containing the IKVAV sequence of laminin perturb neurite outgrowth of peripheral neurons on immature spinal cord substrata [J].