Location of fluorotryptophan sequestered in an amphiphilic nanoparticle by rotational-echo double-resonance NMR

被引：23

作者：

Baugher, AH ^{[1
]}

Goetz, JM ^{[1
]}

McDowell, LM ^{[1
]}

Huang, HY ^{[1
]}

Wooley, KL ^{[1
]}

Schaefer, J ^{[1
]}

机构：

[1] Washington Univ, Dept Chem, St Louis, MO 63130 USA

来源：

BIOPHYSICAL JOURNAL | 1998年 / 75卷 / 05期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0006-3495(98)77702-1

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Rotational-echo double-resonance (REDOR) C-13 NMR spectra (with F-19 dephasing) have been obtained of 6-fluorotryptophan complexed by a polymeric amphiphilic nanosphere consisting of a polystyrene core covalently attached to a poly(acrylic acid)-polyacrylamide shell. The REDOR spectra show that aromatic carbons from the polystyrene core and oxygenated carbons in the poly(acrylic acid)-polyacrylamide shell are both proximate to the F-19 of 6-fluorotryptophan. Molecular modeling restrained by distances inferred from the REDOR spectra suggests that all of the 6-fluorotryptophans are in the shell but within 10 Angstrom of the core-shell interface.

引用

页码：2574 / 2576

页数：3

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