Reaction of cysteine and glutathione (GSH) at the freshly fractured quartz surface: A possible role in silica-related diseases?

被引:35
作者
Fenoglio, I
Fonsato, S
Fubini, B
机构
[1] Univ Turin, IFM, Dept Chem, Dipartimento Chim Inorgan Chim Fis & Chim Mat, I-10125 Turin, Italy
[2] Univ Turin, Interdept Ctr G Scansetti Studies Asbestos & Tox, I-10125 Turin, Italy
关键词
quartz; Thiols; GSH; cysteine; silica toxicity; grinding; particle derived radicals; sulphur centred radicals; free radicals;
D O I
10.1016/S0891-5849(03)00398-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The reactivity of quartz dusts towards glutathione (GSH) and cysteine (Cys) has been investigated. Cys and GSH react, without being adsorbed (UV-Vis spectroscopy), with commercial quartz dusts in an exposed surface-dependent way, but not with amorphous silica. GSH and Cys have been contacted with freshly ground quartz (agate jar QZg-a and steel jar QZg-s) and quartz heated in air at 500degreesC (QZs-500) and with a dust generated from a purified quartz (99.9999%) to detect the nature of the reacting surface sites. With both GSH and Cys, the highest reactivity was found on the particles ground in a steel jar, while pure quartz was fully inactive. Detection of the radical GS(.) (spin trapping) suggests a radical mechanism of oxidation to disulphide onto surface-bound iron traces, more abundant on QZg-s and absent on the pure quartz. Oxidation of thiol groups occurs at surface sites different from those involved in the homolytic rupture of a C-H bond. Both reactions are more pronounced on freshly ground samples, but the C-H rupture takes place at silicon-based surface radicals and Fe2+ centers, while oxidation of GSH and Cys requires Fe3+ centers. As all commercial quartz dusts contain surface iron as an impurity, depletion of extracellular or intracellular GSH may contribute to the oxidative damage caused by particle-derived and cell-derived reactive oxygen species. (C) 2003 Elsevier Inc.
引用
收藏
页码:752 / 762
页数:11
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