Pyrolysis/gas chromatography/mass spectrometry as a useful technique to evaluate the ligninolytic action of streptomycetes on wheat straw

被引:30
作者
Hernández, M
Hernández-Coronado, MJ
Montiel, MD
Rodríguez, J
Pérez, MI
Bocchini, P
Galletti, GC
Arias, ME
机构
[1] Univ Alcala de Henares, Fac Farm, Dept Microbiol & Parasitol, E-28871 Alcala De Henares, Spain
[2] CNR, CSFM, I-40126 Bologna, Italy
关键词
streptomycetes; pyrolysis/gas chromatography/mass spectrometry; lignocellulose degradation; enzymes;
D O I
10.1016/S0165-2370(00)00194-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Pyrolysis/gas chromatography/mass spectrometry (Py/GC/MS) was used to elucidate whether the chemical modifications produced by streptomycetes on wheat straw are related with the lignocellulose degrading enzymes produced by the microorganism on such substrate. Thus, Streptomyces sp. UAH 47 was grown on wheat straw under solid-state fermentation and periodically the major solubilisation product of this substrate (lignin-carbohydrate complex) and the residual wheat straw were pyrolysed. In parallel, different enzyme activities related with lignocellulose degradation were evaluated. The characteristic pyrolysis products detected by GC/MS from both solubilised lignocarbohydrate complex and residual wheat straw demonstrated an oxidative action of the microorganism on the lignin. Either oxidation or oxidation followed by cleavage of the C3 alkyl chain, from S and G units, mainly at the C alpha and C beta position could be detected. A correlation between the oxidation degree of lignin and both L-3,4-dihydroxyphenylalanine and 2,6-dimethoxyphenol peroxidases produced by the strain can be inferred. Moreover, a liberation of ferulic and p-coumaric acids from the substrate was detected by HPLC, which probably involves the action of ferulic and coumaric acid esterases secreted by the microorganism. These enzymes could be partially responsible for the different pattern in the relative area of 4-vinylguaiacol and 4-vinylphenol detected in all samples along the incubation time. At last, the pyrolysis data suggest a straight correlation between the relative area of the compounds derived from carbohydrates and the pattern of hemicellulolytic and cellulolytic enzymes produced by the strain. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:539 / 551
页数:13
相关论文
共 17 条
[1]   Solubilisation and mineralisation of [C-14]lignocellulose from wheat straw by Streptomyces cyaneus CECT 3335 during growth in solid-state fermentation [J].
Berrocal, MM ;
Rodriguez, J ;
Ball, AS ;
PerezLeblic, MI ;
Arias, ME .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1997, 48 (03) :379-384
[2]   LIGNOCELLULOSE DECOMPOSITION BY SELECTED STREPTOMYCES STRAINS [J].
CRAWFORD, DL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1978, 35 (06) :1041-1045
[3]   LIGNIN DEGRADATION BY STREPTOMYCES-VIRIDOSPORUS - ISOLATION AND CHARACTERIZATION OF A NEW POLYMERIC LIGNIN DEGRADATION INTERMEDIATE [J].
CRAWFORD, DL ;
POMETTO, AL ;
CRAWFORD, RL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 45 (03) :898-904
[4]  
Garcia BL, 1998, FEMS MICROBIOL LETT, V158, P95, DOI 10.1111/j.1574-6968.1998.tb12806.x
[5]  
HERNANDEZ M, 1994, APPL ENVIRON MICROB, V60, P3909
[6]   Chemical characterization and spectroscopic analysis of the solubilization products from wheat straw produced by Streptomyces strains grown in solid-state fermentation [J].
HernandezCoronado, MJ ;
Hernandez, M ;
Centenera, F ;
PerezLeblic, MI ;
Ball, AS ;
Arias, ME .
MICROBIOLOGY-SGM, 1997, 143 :1359-1367
[7]  
Lopez Fernandez C. L., 1995, Journal of Basic Microbiology, V35, P405
[8]   Oxidative degradation of alkali wheat straw lignin by fungal lignin peroxidase, manganese peroxidase and laccase: A comparative study [J].
Martinez-Inigo, MJ ;
Kurek, B .
HOLZFORSCHUNG, 1997, 51 (06) :543-548
[9]   ACTINOMYCETES AS AGENTS OF BIODEGRADATION IN THE ENVIRONMENT - A REVIEW [J].
MCCARTHY, AJ ;
WILLIAMS, ST .
GENE, 1992, 115 (1-2) :189-192
[10]   PYROLYSIS-GC-MS CHARACTERIZATION OF FORAGE MATERIALS [J].
RALPH, J ;
HATFIELD, RD .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1991, 39 (08) :1426-1437