Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species

被引:131
作者
SzewcMcFadden, AK
Kresovich, S
Bliek, SM
Mitchell, SE
McFerson, JR
机构
[1] UNIV GEORGIA, PLANT GENET RESOURCES CONSERVAT UNIT, USDA ARS, GRIFFIN, GA 30223 USA
[2] CORNELL UNIV, PLANT GENET RESOURCES UNIT, USDA ARS, GENEVA, NY 14456 USA
关键词
DNA marker; genetic analysis; genetic diversity; genotyping; microsatellite;
D O I
10.1007/BF00417944
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The application of simple sequence repeat (SSR) genotyping for the characterization of genetic variation in crop plants has been hindered bq ready access to useful primer pairs and potentially limited conservation of the repeat sequences among related species. In this phase of work, we report on the identification and characterization of SSRs that are conserved in Brassica napus L. (rape seed) and its putative progenitors, B. oleracea L. (cabbage, and related vegetable types) and B. rapa (vegetable and oil types). Approximately 140 clones from a size-fractionated genomic library of B. napus were sequenced, and primer pairs were designed for 21 dinucleotide SSRs. Seventeen primer pairs amplified products in the three species and. among these, 13 detected variation between and within species. Unlike findings on SSR information content in human no relationship could be established between the number of tandem repeats within the target sequence and heterozygosity. All primer pairs have been designed to work under identical amplification conditions: therefore: single-reaction, multiplex polymerase chain reaction (PCR) with these SSRs is possible. Once moderate numbers of primer pairs are accessible to the user community, SSR genotyping may provide a useful method for the characterization, conservation, and utilization of agricultural crop diversity.
引用
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页码:534 / 538
页数:5
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