Heterogeneity of Helicobacter pylori cag genotypes in experimentally infected mice

被引:18
作者
Sozzi, M
Crosatti, M
Kim, SK
Romero, J
Blaser, MJ
机构
[1] CRO, Div Gastroenterol & Digest Endoscopy, I-33081 Aviano, Italy
[2] Vanderbilt Univ, Div Infect Dis, Nashville, TN USA
[3] NYU, Sch Med, Dept Med & Microbiol, New York, NY USA
关键词
cag genotype; cagA gene; deletion; mouse; Helicobacter pylori;
D O I
10.1111/j.1574-6968.2001.tb10828.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Our aim was to assess whether the Helicobacter pylori population recovered from experimentally infected mice show heterogeneity in cag genotypes. Wild-type FVB/N mice were challenged with strain Hp I and sacrificed 8 weeks later. Direct PCR on gastric tissue was performed using primers for glmM and cagA, and for these two genes and for cagE and virB11 using DNA from the infecting and the emerging strains. The gastric tissues of two of five mice were PCR+ for glmM but not cagA. For the infecting strain, the PCRs for all four genes studied were strongly positive, but the sweeps from the emerging strains from both mice gave weaker signals for cagA and cagE. Examination of single colonies showed reduced or absent signals for cagA and cagE in relation to glmM and virB11. Serial dilution PCR of sweep isolates from the mice showed a 10- to 100-fold decrease in cagA signal compared to the infecting strain. The decrease of cagA and cagE, but not vif-B11, amplification and lack of cagA hybridization in Southern blots indicates a selective loss of the right half of the cag island during murine infection. This phenomenon is consistent with host-induced adaptive changes of cag genotype in the population of colonizing H. pylori cells. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.
引用
收藏
页码:109 / 114
页数:6
相关论文
共 24 条
  • [1] Analyses of the cag pathogenicity island of Helicobacter pylori
    Akopyants, NS
    Clifton, SW
    Kersulyte, D
    Crabtree, JE
    Youree, BE
    Reece, CA
    Bukanov, NO
    Drazek, ES
    Roe, BA
    Berg, DE
    [J]. MOLECULAR MICROBIOLOGY, 1998, 28 (01) : 37 - 53
  • [2] DNA DIVERSITY AMONG CLINICAL ISOLATES OF HELICOBACTER-PYLORI DETECTED BY PCR-BASED RAPD FINGERPRINTING
    AKOPYANZ, N
    BUKANOV, NO
    WESTBLOM, TU
    KRESOVICH, S
    BERG, DE
    [J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (19) : 5137 - 5142
  • [3] Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori
    Alm, RA
    Ling, LSL
    Moir, DT
    King, BL
    Brown, ED
    Doig, PC
    Smith, DR
    Noonan, B
    Guild, BC
    deJonge, BL
    Carmel, G
    Tummino, PJ
    Caruso, A
    Uria-Nickelsen, M
    Mills, DM
    Ives, C
    Gibson, R
    Merberg, D
    Mills, SD
    Jiang, Q
    Taylor, DE
    Vovis, GF
    Trost, TJ
    [J]. NATURE, 1999, 397 (6715) : 176 - 180
  • [4] MOSAICISM IN VACUOLATING CYTOTOXIN ALLELES OF HELICOBACTER-PYLORI - ASSOCIATION OF SPECIFIC VACA TYPES WITH CYTOTOXIN PRODUCTION AND PEPTIC-ULCERATION
    ATHERTON, JC
    CAO, P
    PEEK, RM
    TUMMURU, MKR
    BLASER, MJ
    COVER, TL
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (30) : 17771 - 17777
  • [5] Blaser MJ, 1998, HELICOBACTER PYLORI, P43
  • [6] Host specificity of Helicobacter pylori strains and host responses in experimentally challenged nonhuman primates
    Dubois, A
    Berg, DE
    Incecik, ET
    Fiala, N
    Heman-Ackah, LM
    Del Valle, J
    Yang, MQ
    Wirth, HP
    Perez-Perez, GI
    Blaser, MJ
    [J]. GASTROENTEROLOGY, 1999, 116 (01) : 90 - 96
  • [7] cagA positive and negative Helicobacter pylori strains are simultaneously present in the stomach of most patients with non-ulcer dyspepsia:: relevance to histological damage
    Figura, N
    Vindigni, C
    Covacci, A
    Presenti, L
    Burroni, D
    Vernillo, R
    Banducci, T
    Roviello, F
    Marrelli, D
    Biscontri, M
    Kristodhullu, S
    Gennari, C
    Vaira, D
    [J]. GUT, 1998, 42 (06) : 772 - 778
  • [8] Population genetic analysis of Helicobacter pylori by multilocus enzyme electrophoresis: Extensive allelic diversity and recombinational population structure
    Go, MF
    Kapur, V
    Graham, DY
    Musser, JM
    [J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (13) : 3934 - 3938
  • [9] Metronidazole resistance in Helicobacter pylori is due to null mutations in a gene (rdxA) that encodes an oxygen-insensitive NADPH nitroreductase
    Goodwin, A
    Kersulyte, D
    Sisson, G
    van Zanten, SJOV
    Berg, DE
    Hoffman, PS
    [J]. MOLECULAR MICROBIOLOGY, 1998, 28 (02) : 383 - 393
  • [10] Epithelial attachment alters the outcome of Helicobacter pylori infection
    Guruge, JL
    Falk, PG
    Lorenz, RG
    Dans, M
    Wirth, HP
    Blaser, MJ
    Berg, DE
    Gordon, JI
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (07) : 3925 - 3930