VIP inhibits Porphyromonas gingivalis LPS-induced immune responses in human monocytes

被引：32

作者：

Foster, N ^{[1
]}

Cheetham, J ^{[1
]}

Taylor, JJ ^{[1
]}

Preshaw, PM ^{[1
]}

机构：

[1] Univ Newcastle Upon Tyne, Sch Dent Sci, Oral Microbiol & Host Responses Grp, Newcastle Upon Tyne NE2 4BW, Tyne & Wear, England

来源：

JOURNAL OF DENTAL RESEARCH | 2005年 / 84卷 / 11期

关键词：

monocyte; VIP; Porphyromonas gingivalis; LPS;

D O I：

10.1177/154405910508401106

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Lipopolysaccharide (LPS) from the Gram-negative pathogen Porphyromonas gingivalis (Pg) stimulates cytokine secretion in immune cells, and thereby initiates the inflammation associated with periodontitis. Modulation of pro-inflammatory cytokine activity is a plausible therapeutic target in periodontal disease. Vasoactive intestinal peptide (VIP) has a role in immunoregulation, and has been identified as a molecule with therapeutically beneficial immunosuppressive effects in inflammatory and autoimmune conditions. We aimed to investigate the effect of VIP on immune responses induced by Pg LPS in vitro. VIP (10(-8) M) significantly (P < 0.05) inhibits TNF-alpha production by human monocytic THP1 cells stimulated with Pg LPS. In parallel, we showed that VIP inhibits nuclear translocation of NF kappa B and c-Jun in a time-dependent manner, but does not decrease the expression of CD14 receptors. This is the first report to show the potential of VIP as an immunomodulator of Pg-stimulated inflammatory pathways in human monocytes.

引用

页码：999 / 1004

页数：6

共 28 条

[1] Porphyromonas gingivalis lipopolysaccharide contains multiple lipid a species that functionally interact with both toll-like receptors 2 and 4 [J].