Rice transformation with a senescence-inhibition chimeric gene

被引：12

作者：

Fu, YC

Ding, YY

Liu, XF

Sun, CQ

Cao, SY

Wang, DJ

He, SJ

Wang, XK

Li, LC

Tian, WZ ^{[1
]}

机构：

[1] Chinese Acad Sci, Genet Inst, Beijing 100101, Peoples R China

[2] China Agr Univ, Beijing 100094, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 1998年 / 43卷 / 21期

关键词：

rice; specific promoter; IPT gene; senescence inhibition; biolistic transformation;

D O I：

10.1007/BF02883378

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A senescence-inhibition chimeric gene containing the specific promoter of SAG(12) and IPT gene was transferred into rice with the biolistic method. Results of PCR, Dot blotting and Southern blotting indicated that the chimeric gene had been integrated into rice genome. Analyses of GUS activity and cytokinin content in transgenic plants of rice and the observation of T-1 generation plant at grain formation stage indicated that the foreign gene was expressed.

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收藏

页码：1810 / 1815

页数：6

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