Murine class I major histocompatibility complex H-2Dd:: expression, refolding and crystallization

被引：26

作者：

Achour, A ^{[1
]}

Harris, RA

Persson, K

Sundbäck, J

Sentman, CL

Schneider, G

Lindqvist, Y

Kärre, K

机构：

[1] Karolinska Inst, Microbiol & Tumorbiol Ctr, Stockholm, Sweden

[2] Karolinska Hosp, Ctr Mol Med, S-10401 Stockholm, Sweden

[3] Umea Univ, Ctr Mol Pathogenesis, Umea, Sweden

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷

关键词：

D O I：

10.1107/S0907444998005265

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A truncated soluble form of the murine class I major histocompatibility antigen complex H-2D(d) was cloned using an Escherichia coli based system. It was expressed, refolded in vitro and crystallized in a complex with murine beta(2) microglobulin and the peptide RGPGRAFVTI from the V3-loop of the gp160 HIV-1 protein. Crystals belonging to the space group P2(1)2(1)2(1) with cell dimensions a = 51.3, b = 92.5, c = 108.8 Angstrom were obtained using two different crystallization conditions. The crystals contain one complex per asymmetric unit and diffract to at least 2.4 Angstrom resolution.

引用

页码：260 / 262

页数：3

共 31 条

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