Detection of anthrax vaccine virulence factors by polymerase chain reaction

被引：56

作者：

Fasanella, A

Losito, S

Trotta, T

Adone, R

Massa, S

Ciuchini, F

Chiocco, D

机构：

[1] Ist Zooprofilatt Sperimentale Puglia & Basilicata, I-71100 Foggia, Italy

[2] Ist Super Sanita, Lab Med Vet, I-00161 Rome, Italy

[3] Univ Foggia, Fac Agr, Ist Prod & Tecnol Alimentari, Foggia, Italy

来源：

VACCINE | 2001年 / 19卷 / 30期

关键词：

anthrax; PCR; vaccine;

D O I：

10.1016/S0264-410X(01)00159-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In Italy, an attenuated Bacillus anthracis strain, named `Carbosap', is used for immunization against ovine and bovine anthrax. Analysis on `Carbosap', Sterne vaccine strain F34 and Pasteur vaccine strain SS104, were performed using primers specific for the sequences, encoding the toxic factors, located on plasmids pXO1 and pXO2 and primers specific for the chromosome. The results obtained from polymerase chain reaction (PCR) assay revealed the presence of both plasmids pXO1 and pXO2 in `Carbosap' strain. This study showed that the `Carbosap' vaccine strain has a different plasmid pattern in comparison to Pasteur vaccine strain SS104 and Sterne vaccine strain F34. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：4214 / 4218

页数：5

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