Phospholipase C cDNAs from sponge and hydra: antiquity of genes involved in the inositol phospholipid signaling pathway

被引：48

作者：

Koyanagi, M ^{[1
]}

Ono, K ^{[1
]}

Suga, H ^{[1
]}

Iwabe, N ^{[1
]}

Miyata, T ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Sci, Dept Biophys, Kyoto 6068502, Japan

来源：

FEBS LETTERS | 1998年 / 439卷 / 1-2期

关键词：

phospholipase C; protein kinase Cc; gene duplication; phylogenetic tree; evolution; hydra; sponge;

D O I：

10.1016/S0014-5793(98)01339-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To know whether or not the set of genes involved in the inositol phospholipid signaling pathway already existed in the early evolution of animals, me carried out cloning of cDNAs encoding phospholipase Cs (PLCs) from Ephydatia fluviatilis (freshwater sponge) and Hydra magnipapillata strain 105 (hydra), We isolated two PLC cDNAs, PLC-PS and PLC-gamma S, from sponge and three cDNAs, PLC-beta H1, PLC-beta H2, and PLC-delta H, from hydra, From the domain organization and the divergence pattern in the PLC family tree, the sponge PLC-PS and PLC-gamma S and the hydra PLC-GH are possibly homologous to the vertebrate PLC-beta, PLC-gamma and PLC-delta subtypes, respectively. A detailed phylogenetic analysis suggests that the hydra PLC-beta H1 and PLC-beta H2 are homologs of the vertebrate PLC-beta 1/2/3/ Drosophila PLC21 and the vertebrate PLC-beta 4/Drosophila norpA, respectively. A phylogenetic analysis of the PLC family and the protein kinase C (PKC) family, together with that of the C protein alpha subunit (G alpha) family, revealed that the origin of the set of genes G alpha q, PLC, PKC involved in the inositol phospholipid signaling pathway is very old, going back to dates before the parazoan-eumetazoan split, the earliest branching among extant animal phyla, (C) 1998 Federation of European Biochemical Societies.

引用

页码：66 / 70

页数：5

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