Use of real-time quantitative PCR to detect Chlamydophila felis infection

被引:34
作者
Helps, C [1 ]
Reeves, N [1 ]
Tasker, S [1 ]
Harbour, D [1 ]
机构
[1] Univ Bristol, Dept Vet Clin Sci, Div Mol & Cellular Biol, Bristol BS40 5DU, Avon, England
关键词
D O I
10.1128/JCM.39.7.2675-2676.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A real-time PCR assay was developed to detect and quantify Chlamdophila felis infection of cats. The assay uses a molecular beacon to specifically identify the major outer membrane protein gene, is highly reproducible, and is able to detect fewer than 10 genomic copies.
引用
收藏
页码:2675 / 2676
页数:2
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