Genetic transfer of large DNA inserts to designated loci of the Bacillus subtilis 168 genome

被引：17

作者：

Itaya, M ^{[1
]}

机构：

[1] Mitsubishi Kasei Inst Life Sci, Machida, Tokyo 1948511, Japan

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 03期

关键词：

D O I：

10.1128/JB.181.3.1045-1048.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

It was found that contiguous DNA segments of up to 50 kb can be transferred between Bacillus subtilis genomes when a sufficient length of the flanking genomic region is provided for homologous recombination, although the efficiency of transfer was reduced as the insert size increased. Inserts were translocated to different loci, where appropriate integration sites were created.

引用

页码：1045 / 1048

页数：4

共 23 条

[1] EFFICIENCY OF HOMOLOGOUS INTERMOLECULAR RECOMBINATION AT DIFFERENT LOCATIONS ON THE BACILLUS-SUBTILIS CHROMOSOME [J].

BISWAS, I ;

VAGNER, V ;

EHRLICH, SD .

JOURNAL OF BACTERIOLOGY, 1992, 174 (17) :5593-5596

[2] SEPARATION OF LARGE DNA-MOLECULES BY CONTOUR-CLAMPED HOMOGENEOUS ELECTRIC-FIELDS [J].

CHU, G ;

VOLLRATH, D ;

DAVIS, RW .

SCIENCE, 1986, 234 (4783) :1582-1585

[3] GENETIC EXCHANGE AND EVOLUTIONARY DIVERGENCE IN PROKARYOTES [J].

COHAN, FM .

TRENDS IN ECOLOGY & EVOLUTION, 1994, 9 (05) :175-180

[4]

Cutting S. M., 1990, MOL BIOL METHODS BAC

[5] Microbial genomes opened up [J].

Doolittle, RF .

NATURE, 1998, 392 (6674) :339-342

[6] GENETIC COMPETENCE IN BACILLUS-SUBTILIS [J].

DUBNAU, D .

MICROBIOLOGICAL REVIEWS, 1991, 55 (03) :395-424

[7] Binding and transport of transforming DNA by Bacillus subtilis: The role of type-IV pilin-like proteins - A review [J].

Dubnau, D .

GENE, 1997, 192 (01) :191-198

[8] THE BACILLUS-SUBTILIS CHROMOSOME [J].

HENNER, DJ ;

HOCH, JA .

MICROBIOLOGICAL REVIEWS, 1980, 44 (01) :57-82

[9] Predicted and unsuspected alterations of the genomes structure of genetically defined Bacillus subtilis 168 strains [J].

Itaya, M ;

Tanaka, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1997, 61 (01) :56-64

[10] TOWARD A BACTERIAL GENOME TECHNOLOGY - INTEGRATION OF THE ESCHERICHIA-COLI PROPHAGE LAMBDA-GENOME INTO THE BACILLUS-SUBTILIS 168-CHROMOSOME [J].

ITAYA, M .

MOLECULAR & GENERAL GENETICS, 1995, 248 (01) :9-16

← 1 2 3 →