ORESTES are enriched in rare exon usage variants affecting the encoded proteins

被引：9

作者：

Sakabe, NJ

de Souza, JES

Galante, PAF

de Oliveira, PSL

Passetti, F

Brentani, H

Osório, EC

Zaiats, AC

Leerkes, MR

Kitajima, JP

Brentani, RR

Strausberg, RL

Simpson, AJG

de Souza, SJ

机构：

[1] Ludwig Inst Canc Res, Sao Paulo Branch, BR-01509010 Sao Paulo, Brazil

[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo, Brazil

[3] Univ Sao Paulo, Curso Posgrad Bioinformat, Sao Paulo, Brazil

[4] Univ Sao Paulo, Inst Coracao, Sao Paulo, Brazil

[5] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, Brazil

[6] NCI, Bethesda, MD 20892 USA

来源：

COMPTES RENDUS BIOLOGIES | 2003年 / 326卷 / 10-11期

关键词：

alternative splicing; alternative exon usage; bioinformatics; genome mapping; ORESTES; human transcriptome;

D O I：

10.1016/j.crvi.2003.09.027

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A significant fraction of the variability found in the human transcriptome is due to alternative splicing, including alternative exon usage (AEU), intron retention and use of cryptic splice sites. We present a comparison of a large-scale analysis of AEU in the human transcriptome through genome mapping of Open Reading Frame ESTs (ORESTES) and conventional ESTs. It is shown here that ORESTES probe low abundant messages more efficiently. In addition, most of the variants detected by ORESTES affect the structure of the corresponding proteins. (C) 2003 Academie des sciences. Published by Elsevier SAS. All rights reserved.

引用

页码：979 / 985

页数：7

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