Intramuscular injection of plasmid DNA encoding cottontail rabbit papillomavirus E1, E2, E6 and E7 induces T cell-mediated but not humoral immune responses in rabbits

被引：35

作者：

Han, R ^{[1
]}

Reed, CA ^{[1
]}

Cladel, NM ^{[1
]}

Christensen, ND ^{[1
]}

机构：

[1] Penn State Univ, Coll Med, Dept Pathol, Jake Gittlen Canc Res Inst, Hershey, PA 17033 USA

来源：

VACCINE | 1999年 / 17卷 / 11-12期

关键词：

DNA vaccine; papillomavirus; antiviral immunity;

D O I：

10.1016/S0264-410X(98)00356-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To test the efficacy of genetic vaccination against papillomavirus infection, plasmid DNA encoding cottontail rabbit papillomavirus (CRPV) E1, E2, E6, E7 or without insert were intramuscularly injected into five groups of rabbits. Peripheral blood mononuclear cells (PBMCs) showed specific proliferation upon in vitro stimulation with El, E7, E6 or E7 proteins in a majority of vaccinated rabbits but Western blot analysis did not detect antibodies specific for these viral proteins in rabbit serum. All rabbits grew papillomas after virus challenge and none of the rabbits showed systemic papilloma regression. These observations showed that intramuscular injection of plasmid DNA encoding CRPV E1, E2, E6 or E7 induced CD4(+) T cell-mediated but not humoral immune responses, and did not result in the protection of rabbits from virus infection. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：1558 / 1566

页数：9

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