Conversion of indene to cis-(1S),(2R)-indandiol by mutants of Pseudomonas putida F1

被引：25

作者：

Connors, N ^{[1
]}

Prevoznak, R ^{[1
]}

Chartrain, M ^{[1
]}

Reddy, J ^{[1
]}

Singhvi, R ^{[1
]}

Patel, Z ^{[1
]}

Olewinski, R ^{[1
]}

Salmon, P ^{[1
]}

Wilson, J ^{[1
]}

Greasham, R ^{[1
]}

机构：

[1] MERCK RES LABS,DEPT BIOPROC RES & DEV,RAHWAY,NJ

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 1997年 / 18卷 / 06期

关键词：

biocatalysis; dioxygenase; Pseudomonas putida; cis-(1S),(2R)-indandiol; indene;

D O I：

10.1038/sj.jim.2900402

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two mutation and selection methods were used to isolate mutants of Pseudomonas putida F1 which convert indene to cis-(1S),(2R)-indandiol in a toluene-independent fashion, Using soybean or silicone oil as a second phase to deliver indene to the culture, cis-(1S),(2R)-indandiol, cis-(1R),(2S)-indandiol, 1,2-indenediol (or the keto-hydroxy indan tautomer), and the monooxygenation products 1-indenol and 1-indanone were produced from indene as a function of time, Similarly the enantiomeric excess of the cis-(1S),(2R)-indandiol produced also increased with increasing time, In addition, mutants were isolated which produced cis-(1S),(SR)-indandiol of lower optical purity which corresponded to reduced levels of 1,2-indenediol. These data suggest this toluene dioxygenase produces cis-(1S),(2R)-indandiol of low optical purity and that cis-glycol dehydrogenase plays a role in resolving the two cis-1,2-indandiol enantiomers.

引用

页码：353 / 359

页数：7

共 17 条

[1] A BIOTECH ROUTE TO POLYPHENYLENE [J].