Stable RK2-derived cloning vectors for the analysis of gene expression and gene function in gram-negative bacteria

被引：107

作者：

Dombrecht, B ^{[1
]}

Vanderleyden, J ^{[1
]}

Michiels, J ^{[1
]}

机构：

[1] Katholieke Univ Leuven, FA Janssens Lab Genet, B-3001 Heverlee, Belgium

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2001年 / 14卷 / 03期

关键词：

Azospirillum brasilense Sp245; post-segregational killing; pTR102; Rhizobium etli CNPAF512; y4dLM; y4mFE;

D O I：

10.1094/MPMI.2001.14.3.426

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The construction of several stable RK2-derived cloning vectors for the analysis of gene expression and function in gram-negative bacteria is reported. Plasmid stability is Conferred by the RK2 par locus or by insertion of the spsAB or spsCD symbiotic plasmid stability loci from pNGR234a of Rhizobium sp. NGR234. The vectors carry multiple cloning sites with protection against read-through transcriptional activity of vector sequences. Vector derivatives with the constitutive nptII promoter or a promoter-less gusA gene are suitable for the study of gene function or regulation in bacteria.

引用

页码：426 / 430

页数：5

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