Structural basis for the catalytic mechanism of phosphothreonine lyase

被引：37

作者：

Chen, Linjie ^{[1
,2
,3
]}

Wang, Huayi ^{[1
,2
,3
]}

Zhang, Jie ^{[1
]}

Gu, Lichuan ^{[4
]}

Huang, Niu ^{[1
,5
]}

Zhou, Jian-Min ^{[1
]}

Chai, Jijie ^{[1
]}

机构：

[1] Natl Inst Biol Sci, Beijing 102206, Peoples R China

[2] Beijing Union Med Coll, Beijing 100730, Peoples R China

[3] Grad Program Chinese Acad Med Sci, Beijing 100730, Peoples R China

[4] Shandong Univ, State Key Microbial Technol, Jinan 250100, Peoples R China

[5] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2008年 / 15卷 / 01期

关键词：

D O I：

10.1038/nsmb1329

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Salmonella SpvC belongs to a new enzyme family designated phosphothreonine lyases that irreversibly inactivate mitogen-activated protein kinases. The crystal structure of SpvC reported here reveals that the two phosphorylated residues in the substrate peptide predominantly mediate its recognition by SpvC. Substrate-induced conformational changes in SpvC sequester the phosphothreonine in a completely solvent-free environment, preventing the hydrolysis of the phosphate group and facilitating the elimination reaction.

引用

收藏

页码：101 / 102

页数：2

相关论文

共 12 条

[1] Pathogen recognition and innate immunity [J].

Akira, S ;

Uematsu, S ;

Takeuchi, O .

CELL, 2006, 124 (04) :783-801

[2] An injected bacterial effector targets chromatin access for transcription factor NF-κB to alter transcription of host genes involved in immune responses [J].

Arbibe, Laurence ;

Kim, Dong Wook ;

Batsche, Eric ;

Pedron, Thierry ;

Mateescu, Bogdan ;

Muchardt, Christian ;

Parsot, Claude ;

Sansonetti, Philippe J. .

NATURE IMMUNOLOGY, 2007, 8 (01) :47-56

[3] The many paths to p38 mitogen-activated protein kinase activation in the immune system [J].

Ashwell, Jonathan D. .

NATURE REVIEWS IMMUNOLOGY, 2006, 6 (07) :532-540

[4] Are innate immune signaling pathways in plants and animals conserved? [J].

Ausubel, FM .

NATURE IMMUNOLOGY, 2005, 6 (10) :973-979

[5] Bacterial strategies for overcoming host innate and adaptive immune responses [J].

Hornef, MW ;

Wick, MJ ;

Rhen, M ;

Normark, S .

NATURE IMMUNOLOGY, 2002, 3 (11) :1033-1040

[6] Yeast functional genomic screens lead to identification of a role for a bacterial effector in innate immunity regulation [J].

Kramer, Roger W. ;

Slagowski, Naomi L. ;

Eze, Ngozi A. ;

Giddings, Kara S. ;

Morrison, Monica F. ;

Siggers, Keri A. ;

Starnbach, Michael N. ;

Lesser, Cammie F. .

PLOS PATHOGENS, 2007, 3 (02) :179-190

[7] The phosphothreonine lyase activity of a bacterial type III effector family [J].

Li, Hongtao ;

Xu, Hao ;

Zhou, Yan ;

Zhang, Jie ;

Long, Chengzu ;

Li, Shuqin ;

Chen, She ;

Zhou, Jian-Min ;

Shao, Feng .

SCIENCE, 2007, 315 (5814) :1000-1003

[8] Virulence plasmid-borne spvB and spvC genes can replace the 90-kilobase plasmid in conferring virulence to Salmonella enterica serovar Typhimurium in subcutaneously inoculated mice [J].

Matsui, H ;

Bacot, CM ;

Garlington, WA ;

Doyle, TJ ;

Roberts, S ;

Gulig, PA .

JOURNAL OF BACTERIOLOGY, 2001, 183 (15) :4652-4658

[9] Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation [J].

Mukherjee, Sohini ;

Keitany, Gladys ;

Li, Yan ;

Wang, Yong ;

Ball, Haydn L. ;

Goldsmith, Elizabeth J. ;

Orth, Kim .

SCIENCE, 2006, 312 (5777) :1211-1214

[10] A LIGAND-INDUCED CONFORMATIONAL CHANGE IN THE YERSINIA PROTEIN-TYROSINE-PHOSPHATASE [J].

SCHUBERT, HL ;

FAUMAN, EB ;

STUCKEY, JA ;

DIXON, JE ;

SAPER, MA .

PROTEIN SCIENCE, 1995, 4 (09) :1904-1913