Thrombopoietin production in wild-type and interleukin-6 knockout mice with acute inflammation

被引:29
作者
Burmester, H
Wolber, EM
Freitag, P
Fandrey, J
Jelkmann, W [1 ]
机构
[1] Univ Luebeck, Inst Physiol, D-23538 Lubeck, Germany
[2] Beth Israel Deaconess Med Ctr, Div Hematol & Oncol, Harvard Inst Med 1043, Boston, MA 02215 USA
[3] Univ Duisburg Essen, Inst Physiol, Essen, Germany
关键词
D O I
10.1089/jir.2005.25.407
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Clinical and laboratory studies indicate that thrombopoietin (TPO) gene expression increases during inflammation. To clarify the role of interleukin 6 (IL-6) in this process, blood cell counts, plasma TPO concentrations, and hepatic and renal TPO mRNA levels were investigated in wild-type and IL-6 knockout mice, with sterile abscesses produced by subcutaneous injection of turpentine oil. Treatment did not cause a change in blood cell counts during the 72 h period of observation. The numbers of thrombocytes and erythrocytes were slightly lower in the IL-6 knockout mice than in the wild-type littermates under all conditions. Plasma IL-6 and TPO concentrations increased on turpentine injection only in the wild-type mice. In addition, turpentine treatment of these caused an increase in hepatic TPO mRNA levels as assessed by competitive polymerase chain reaction (RT-PCR) and real-time PCR, whereas renal TPO mRNA levels were unaltered. TPO mRNA levels did not increase in the livers of IL-6 knockout mice on turpentine treatment. These results support the concept that TPO behaves like an acute-phase protein in that its synthesis is induced by IL-6 in the liver.
引用
收藏
页码:407 / 413
页数:7
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