Effects of leukemia inhibitory factor and oncostatin M on bone mineral formed in in vitro rat bone-marrow stromal cell culture:: Physicochemical aspects

Leukemia inhibitory factor (LIF) and oncostatin RI (OSM), two pleiotropic cytokines involved in bone remodeling, have both anabolic and catabolic activities. This study analyzed the effects of LIF and OSM on the physicochemical characteristics of mineral phases formed in a rat bone-marrow stromal cell culture model. Stromal cells were cultured for three weeks in the presence of 10(-8) Ri dexamethasone, 50 mu g/mL ascorbic acid and 10 mM Na beta-glycerophosphate with or without 10 ng/ml LIF or OSM. Subsequently, the physicochemical characteristics of the mineralization nodules formed were analyzed by energy dispersive X ray microanalysis (EDX) and Fourier transform-infrared (FT-LR) and FT-Raman spectroscopy. EDX and FT-IR spectroscopy revealed the influence of LIF and OSM on the physicochemical characteristics of mineral phases. FT-Raman spectroscopy showed modifications of the main vibrational modes of the organic matrix. These alterations induced by growth factors could help define new strategies for the prevention and treatment of skeletal disorders, (C) 1998 Academic Press.