Structural Basis of Cyanobacterial Photosystem II Inhibition by the Herbicide Terbutryn

被引:76
作者
Broser, Matthias [2 ]
Gloeckner, Carina [2 ]
Gabdulkhakov, Azat [1 ]
Guskov, Albert [1 ]
Buchta, Joachim [3 ]
Kern, Jan [2 ]
Mueh, Frank [2 ]
Dau, Holger [3 ]
Saenger, Wolfram [1 ]
Zouni, Athina [2 ]
机构
[1] Free Univ Berlin, Inst Chem & Biochem Kristallog, D-14195 Berlin, Germany
[2] Tech Univ Berlin, Inst Chem, Max Volmer Lab Biophys Chem, D-10623 Berlin, Germany
[3] Free Univ Berlin, Fachbereich Phys, D-14195 Berlin, Germany
关键词
PHOTOSYNTHETIC REACTION-CENTER; OXYGEN-EVOLVING COMPLEX; RHODOPSEUDOMONAS-VIRIDIS; ELECTRON-TRANSFER; BINDING NICHE; MODEL; CHLORIDE; RESISTANCE; ENERGETICS; RESOLUTION;
D O I
10.1074/jbc.M110.215970
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Herbicides that target photosystem II (PSII) compete with the native electron acceptor plastoquinone for binding at the Q(B) site in the D1 subunit and thus block the electron transfer from Q(A) to Q(B). Here, we present the first crystal structure of PSII with a bound herbicide at a resolution of 3.2 angstrom. The crystallized PSII core complexes were isolated from the thermophilic cyanobacterium Thermosynechococcus elongatus. The used herbicide terbutryn is found to bind via at least two hydrogen bonds to the Q(B) site similar to photosynthetic reaction centers in anoxygenic purple bacteria. Herbicide binding to PSII is also discussed regarding the influence on the redox potential of Q(A), which is known to affect photoinhibition. We further identified a second and novel chloride position close to the water-oxidizing complex and in the vicinity of the chloride ion reported earlier (Guskov, A., Kern, J., Gabdulkhakov, A., Broser, M., Zouni, A., and Saenger, W. (2009) Nat. Struct. Mol. Biol. 16, 334-342). This discovery is discussed in the context of proton transfer to the lumen.
引用
收藏
页码:15964 / 15972
页数:9
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