Effect of traY amber mutations on F-plasmid traY promoter activity in vivo

被引：23

作者：

Silverman, PM

Sholl, A

机构：

[1] Prog. in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 19期

关键词：

D O I：

10.1128/jb.178.19.5787-5789.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have examined the effect of the F plasmid TraY protein on tm gene expression in vivo. Expression was assayed as alkaline phosphatase activity in cells containing a traY Phi(traA'-'phoA)hyb operon under traY promoter control. Amber mutations in traY significantly reduced alkaline phosphatase activity. Since nonsense polarity effects were minimal, if they occurred at all, these data provide the first direct evidence that TraY regulates tra gene expression.

引用

页码：5787 / 5789

页数：3

共 29 条

[1] BEGINNING A GENETIC ANALYSIS OF CONJUGATIONAL TRANSFER DETERMINED BY F-FACTOR IN ESCHERICHIA-COLI BY ISOLATION AND CHARACTERIZATION OF TRANSFER-DEFICIENT MUTANTS
ACHTMAN, M
WILLETTS, N
CLARK, AJ
[J]. JOURNAL OF BACTERIOLOGY, 1971, 106 (02) : 529 - &
[2] THE ROLE OF THE PILUS IN RECIPIENT CELL RECOGNITION DURING BACTERIAL CONJUGATION MEDIATED BY F-LIKE PLASMIDS
ANTHONY, KG
SHERBURNE, C
SHERBURNE, R
FROST, LS
[J]. MOLECULAR MICROBIOLOGY, 1994, 13 (06) : 939 - 953
[3] LINKAGE MAP OF ESCHERICHIA-COLI K-12, EDITION-8
BACHMANN, BJ
[J]. MICROBIOLOGICAL REVIEWS, 1990, 54 (02) : 130 - 197
[4] TRAY PROTEINS OF F AND RELATED EPISOMES ARE MEMBERS OF THE ARC AND MNT REPRESSOR FAMILY
BOWIE, JU
SAUER, RT
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 211 (01) : 5 - 6
[5] STRUCTURE OF ARC REPRESSOR IN SOLUTION - EVIDENCE FOR A FAMILY OF BETA-SHEET DNA-BINDING PROTEINS
BREG, JN
VANOPHEUSDEN, JHJ
BURGERING, MJM
BOELENS, R
KAPTEIN, R
[J]. NATURE, 1990, 346 (6284) : 586 - 589
[6] ANALYSIS OF REGULATION OF ESCHERICHIA-COLI ALKALINE-PHOSPHATASE SYNTHESIS USING DELETIONS AND PHI-80 TRANSDUCING PHAGES
BRICKMAN, E
BECKWITH, J
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1975, 96 (02) : 307 - 316
[7] TRAY AND TRAI ARE REQUIRED FOR ORIT-DEPENDENT ENHANCED RECOMBINATION BETWEEN LAC-CONTAINING PLASMIDS AND LAMBDA-PLAC5
CARTER, JR
PORTER, RD
[J]. JOURNAL OF BACTERIOLOGY, 1991, 173 (03) : 1027 - 1034
[8] Chamberlin M., 1982, ENZYMES, V15, P87
[9] CHARACTERIZATION OF AN INVIVO SYSTEM FOR NICKING AT THE ORIGIN OF CONJUGAL DNA TRANSFER OF THE SEX FACTOR-F
EVERETT, R
WILLETTS, N
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1980, 136 (02) : 129 - 150
[10] ANALYSIS OF THE SEQUENCE AND GENE-PRODUCTS OF THE TRANSFER REGION OF THE F-SEX FACTOR
FROST, LS
IPPENIHLER, K
SKURRAY, RA
[J]. MICROBIOLOGICAL REVIEWS, 1994, 58 (02) : 162 - 210

← 1 2 3 →