Effect of traY amber mutations on F-plasmid traY promoter activity in vivo

被引:23
作者
Silverman, PM
Sholl, A
机构
[1] Prog. in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City
关键词
D O I
10.1128/jb.178.19.5787-5789.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have examined the effect of the F plasmid TraY protein on tm gene expression in vivo. Expression was assayed as alkaline phosphatase activity in cells containing a traY Phi(traA'-'phoA)hyb operon under traY promoter control. Amber mutations in traY significantly reduced alkaline phosphatase activity. Since nonsense polarity effects were minimal, if they occurred at all, these data provide the first direct evidence that TraY regulates tra gene expression.
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页码:5787 / 5789
页数:3
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