The essential genome of a bacterium

被引：243

作者：

Christen, Beat ^{[1
]}

Abeliuk, Eduardo ^{[1
,2
]}

Collier, John M. ^{[3
]}

Kalogeraki, Virginia S. ^{[1
]}

Passarelli, Ben ^{[3
,4
]}

Coller, John A. ^{[3
]}

Fero, Michael J. ^{[1
]}

McAdams, Harley H. ^{[1
]}

Shapiro, Lucy ^{[1
]}

机构：

[1] Stanford Univ, Dept Dev Biol, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Elect Engn, Stanford, CA 94305 USA

[3] Stanford Univ, Funct Genom Facil, Stanford, CA 94305 USA

[4] Stanford Univ, Stem Cell Inst, Genome Ctr, Stanford, CA 94305 USA

来源：

MOLECULAR SYSTEMS BIOLOGY | 2011年 / 7卷

基金：

瑞士国家科学基金会;

关键词：

functional genomics; next-generation sequencing; systems biology; transposon mutagenesis; HIGH-THROUGHPUT IDENTIFICATION; GLOBAL TRANSPOSON MUTAGENESIS; CELL-CYCLE; CAULOBACTER-CRESCENTUS; GENETIC CIRCUIT; REPLICATION; ROBUSTNESS; REGULATORS; SYSTEM; KINASE;

D O I：

10.1038/msb.2011.58

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Caulobacter crescentus is a model organism for the integrated circuitry that runs a bacterial cell cycle. Full discovery of its essential genome, including non-coding, regulatory and coding elements, is a prerequisite for understanding the complete regulatory network of a bacterial cell. Using hypersaturated transposon mutagenesis coupled with high-throughput sequencing, we determined the essential Caulobacter genome at 8 bp resolution, including 1012 essential genome features: 480 ORFs, 402 regulatory sequences and 130 non-coding elements, including 90 intergenic segments of unknown function. The essential transcriptional circuitry for growth on rich media includes 10 transcription factors, 2 RNA polymerase sigma factors and 1 anti-sigma factor. We identified all essential promoter elements for the cell cycle-regulated genes. The essential elements are preferentially positioned near the origin and terminus of the chromosome. The high-resolution strategy used here is applicable to high-throughput, full genome essentiality studies and large-scale genetic perturbation experiments in a broad class of bacterial species. Molecular Systems Biology 7: 528; published online 30 August 2011; doi: 10.1038/msb.2011.58

引用

页数：7

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