Cloning of a novel gene specifically expressed in clonal mouse chondroprogenitor-like EC cells, ATDC5

被引：1

作者：

Akiyama, H ^{[1
]}

Hiraki, Y

Shigeno, C

Ito, H

Kawai, J

Konishi, J

Shimizu, Y

Nakamura, T

机构：

[1] Kyoto Univ, Dept Orthopaed Surg, Sakyo Ku, Kyoto 60601, Japan

[2] Kyoto Univ, Dept Mol Interact & Tissue Engn, Sakyo Ku, Kyoto 60601, Japan

[3] Kyoto Univ, Dept Nucl Med & Diagnost Imaging, Calcium Lab, Sakyo Ku, Kyoto 60601, Japan

[4] Kyoto Univ, Inst Frontier Med Sci, Dept Bioartificial Organs, Sakyo Ku, Kyoto 60601, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1999年 / 1444卷 / 02期

基金：

日本学术振兴会;

关键词：

differential display; ATDC5; chondrogenesis; cloning;

D O I：

10.1016/S0167-4781(98)00278-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned a full-length cDNA encoding a novel mouse protein, A-C2, by differential display method using mouse embryonic fibroblast C3H10T1/2 cells and mouse chondroprogenitor-like EC cells, ATDC5. The deduced amino acid sequence of A-C2 consisted of 106 amino acids with no significant homology to the sequences previously reported. Northern blot analysis showed two major bands of 2.1 and 1.8 kb sizes. Expression of A-C2 mRNA was exclusive to ATDC5 cells at their undifferentiated stage. None of ATDC5 cells at their differentiated stage and adult mice tissues examined expressed A-C2 gene. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：291 / 294

页数：4

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