High-throughput identification of substrate specificity for protein kinase by using an improved one-bead-one-compound library approach

被引：21

作者：

Kim, Yun-Gon

Shin, Dong-Sik

Kim, Eun-Mi

Park, Hyung-Yeon

Lee, Chang-Soo

Kim, June-Hyung

Lee, Bon-Su

Lee, Yoon-Sik

Kim, Byung-Gee ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem & Biol Engn, Int Program Biochem Engn & Biotechnol, Seoul 151742, South Korea

[2] Inha Univ, Dept Chem, Inchon 402751, South Korea

[3] Chungnam Natl Univ, Dept Chem & Biol Engn, Taejon 305764, South Korea

[4] Dong A Univ, Dept Chem Engn, Pusan 604714, South Korea

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 28期

关键词：

enzymes; high-throughput screening; mass spectrometry; peptides; substrate specificity;

D O I：

10.1002/anie.200700195

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Graph Presented) Pick and choose: The identification of the substrate specificity of a protein kinase is critical in understanding its role and function in a cellular signal transduction network. A high-throughput platform was developed for the identification of tyrosine kinase substrate specificity by using an improved one-bead-one-compound ladder peptide library and MALDI-TOF MS. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5408 / 5411

页数：4

共 24 条

[1] On-bead fluorescence assay for serine/threonine kinases [J].