Naturalistic activation of barosensitive afferents release substance P in the nucleus tractus solitarius of the cat

The role for substance P (SP) in baroreceptor transmission in the nucleus tractus solitarius (NTS) remains an area of active research. The purpose of the present study was to determine whether naturalistic activation of barosensitive afferent fibers in the glossopharygneal and vagus nerves release SP in the caudal NTS. Experiments were performed on chloralose anesthetized, artificially ventilated and paralyzed cats. A microdialysis probe was stereotaxically positioned unilaterally in the NTS. Dialysate samples were collected and SP-like immunoreactivity (SP-LI) was measured by radioimmunoassay. Barosensitive afferents were mechanically activated by inflation of a balloon catheter positioned in the thoracic aorta at heart level. Graded balloon inflation produced increases in mean arterial pressure (MAP) of 33+/-5 mmHg and 60+/-3 mmHg (P<0.05) and evoked proportional baroreflex decreases in heart rate of 8+/-3 b.p.m. and 19+/-3 b.p.m. (P<0.05). This was accompanied by increases in SP-LI of 16+/-3% and 39+/-8%, respectively (P<0.05). A positive linear relationship was found between changes in MAP and SP-LI (slope=1.73 fmol/<mu>l/mmHg. r(2)=0.62) that was completely abolished following barodenervation. These findings provide evidence that naturalistic activation of pressure-sensitive afferents in the glossopharygneal and vagus nerves release SP in a region of the NTS that receives primary afferent projections from aortic, carotid sinus and cardiac receptors in the cat. (C) 2001 Elsevier Science B.V. All rights reserved.