Genomic DNA amplification from a single bacterium

被引：222

作者：

Raghunathan, A ^{[1
]}

Ferguson, HR ^{[1
]}

Bornarth, CJ ^{[1
]}

Song, WM ^{[1
]}

Driscoll, M ^{[1
]}

Lasken, RS ^{[1
]}

机构：

[1] Mol Staging Inc, New Haven, CT USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 06期

关键词：

D O I：

10.1128/AEM.71.6.3342-3347.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying nonculturable microorganisms.

引用

页码：3342 / 3347

页数：6

共 7 条

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