Short chain oligogalacturonides induce ethylene production and expression of the gene encoding aminocyclopropane 1-carboxylic acid oxidase in tomato plants

被引:69
作者
Simpson, SD
Ashford, DA
Harvey, DJ
Bowles, DJ
机构
[1] Univ York, Dept Biol, Plant Lab, York YO1 5YW, N Yorkshire, England
[2] Univ Oxford, Dept Biochem, Oxford Glycobiol Inst, Oxford OX1 3QU, England
关键词
aminocyclopropane 1-carboxylic acid oxidase; ethylene; oligogalacturonides; tomato; wounding;
D O I
10.1093/glycob/8.6.579
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Oligogalacturonic acids (OGAs), derived from plant cell wall pectin, have been implicated in a number of signal transduction pathways involved in growth, development and defense responses of higher plants. This study investigates the size range of OGAs capable of inducing ethylene synthesis in tomato plants, and demonstrates that in contrast with many other effects, only short chain OGAs are active. Oligomers across a range of DP from 2-15 were separated and purified to homogeneity by QAE-Sephadex anion exchange chromatography using a novel elution system. The OGAs were applied to tomato plants and assayed for their ability to induce ethylene gas release and changes in steady state levels of mRNA encoding the ethylene forming enzyme aminocyclopropane-1-carboxylic acid oxidase (ACO). The study demonstrated that only OGAs in the size range of DP4-6 were active both in eliciting ACO expression and in the production of ethylene.
引用
收藏
页码:579 / 583
页数:5
相关论文
共 39 条
  • [1] OLIGOGALACTURONIDES INHIBIT THE FORMATION OF ROOTS ON TOBACCO EXPLANTS
    BELLINCAMPI, D
    SALVI, G
    DELORENZO, G
    CERVONE, F
    MARFA, V
    EBERHARD, S
    DARVILL, A
    ALBERSHEIM, P
    [J]. PLANT JOURNAL, 1993, 4 (01) : 207 - 213
  • [2] BISHOP PD, 1984, J BIOL CHEM, V259, P3172
  • [3] NEW METHOD FOR QUANTITATIVE-DETERMINATION OF URONIC ACIDS
    BLUMENKR.N
    ASBOEHAN.G
    [J]. ANALYTICAL BIOCHEMISTRY, 1973, 54 (02) : 484 - 489
  • [4] BOUZAYEN M, 1990, PLANTA, V180, P175, DOI 10.1007/BF00193992
  • [5] COMPETITIVE-INHIBITION OF THE AUXIN-INDUCED ELONGATION BY ALPHA-D-OLIGOGALACTURONIDES IN PEA STEM SEGMENTS
    BRANCA, C
    DELORENZO, G
    CERVONE, F
    [J]. PHYSIOLOGIA PLANTARUM, 1988, 72 (03) : 499 - 504
  • [6] PECTIC POLYSACCHARIDES ELICIT CHITINASE ACCUMULATION IN TOBACCO
    BROEKAERT, WF
    PEUMANS, WJ
    [J]. PHYSIOLOGIA PLANTARUM, 1988, 74 (04) : 740 - 744
  • [7] CAMBELL A, 1991, PLANT PHYSIOL, V97, P699
  • [8] CAMBELL A, 1991, PLANT PHYSIOL, V97, P706
  • [9] CERVONE E, 1989, NATO ASI SERIES H, V36, P85
  • [10] HOST-PATHOGEN INTERACTIONS .33. A PLANT PROTEIN CONVERTS A FUNGAL PATHOGENESIS FACTOR INTO AN ELICITOR OF PLANT DEFENSE RESPONSES
    CERVONE, F
    HAHN, MG
    DELORENZO, G
    DARVILL, A
    ALBERSHEIM, P
    [J]. PLANT PHYSIOLOGY, 1989, 90 (02) : 542 - 548