Kinetic characterization of the glycosyltransferase module of Staphylococcus aureus PBP2

被引：44

作者：

Barrett, D

Leimkuhler, C

Chen, L

Walker, D

Kahne, D

Walker, S

机构：

[1] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA

[2] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

[3] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

来源：

JOURNAL OF BACTERIOLOGY | 2005年 / 187卷 / 06期

关键词：

D O I：

10.1128/JB.187.6.2215-2217.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes.

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页码：2215 / 2217

页数：3

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