A note on poly-L-lysine-mediated gene transfer in HeLa cells

被引：11

作者：

Joubert, D

van Zyl, J

Hawtrey, A

Ariatti, M

机构：

[1] Univ Stellenbosch, Sch Med, Dept Pharmacol, ZA-7505 Tygerberg, South Africa

[2] Univ KwaZulu Natal, Dept Biochem, ZA-4000 Durban, South Africa

来源：

DRUG DELIVERY | 2003年 / 10卷 / 03期

关键词：

luciferase DNA; polylysine; transfection;

D O I：

10.1080/713840395

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Poly-L-lysines of chain lengths varying from 70 to 300 residues are shown to bring about luciferase pRSVL DNA uptake and expression in HeLa cells. Transfection was approximately 50% that of the cationic liposome DOTAB. Expression was higher in the presence of chloroquine. Of interest was the fact that luciferase activity depended on the polysine/DNA charge ratio (+ / -). Maximum activity occurred at a charge ratio (+/-) of 3, while at a charge ratio of I (conjugate electrically neutral) activity was much lower. At the higher charge ratios (+/-) of 4 and 5, luciferase activity decreased. The results obtained are discussed.

引用

页码：209 / 211

页数：3

共 11 条

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